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LIGHT BLUE FIXATIVE AND DECALCIFYING SOLUTION – IDEAL FOR BONE AND HARD TISSUE IN HISTOLOGY
INTRODUCTION
In the field of histology, the microscopic analysis of bone
and hard tissue samples is essential for medical research and diagnosis. To achieve accurate results, it is crucial to conduct decalcification of these samples. OsteoCal Mild Blue is the solution you need for efficient and precise decalcification.
THE DECALCIFICATION PROCESS
Decalcification is the process of removing calcium from
bone and hard tissue, allowing for further microscopic
analysis. The duration of decalcification varies based on the sample’s size and density. OsteoCal Mild Blue,
containing inorganic hydrochloric acid, rapidly softens the
tissue and prepares it for analysis. This solution is suitable for bone, teeth, and keratinized tissue samples
such as filiform warts and nails.
PRODUCT DESCRIPTION
– OSTEOCAL MILD BLUE: Light blue fixative and decalcifying solution for bone and hard tissue in histology. Contains formaldehyde and hydrochloric acid.
– Other Compatible Reagents: Explore our range of complementary products, including fixatives, dehydrating
agents, clearing agents, infiltration agents, immersion media, staining reagents, and more.
PREPARING THE SAMPLE FOR DECALCIFICATION
1. Fixation: Ensure the tissue sample is properly fixated.
2. Immerse: Place the tissue sample into OsteoCal Mild Blue
for complete decalcification.
DECALCIFICATION GUIDELINES
Bone, Teeth, Hard Tissue: Duration: 6-8 hours for a 1 x 1 x 0.3 cm bone (e.g., femur).
Mildly Calcified Tissue (e.g., blood vessels): Duration: 30-60 minutes.
Keratinized Tissue (e.g., nails, filiform warts): Duration: 15-60 minutes with the cross-section oriented downward.
ENSURING COMPLETION
Use a needle to puncture an unimportant part of the sample to determine the end of the decalcification process. Incomplete decalcification can be supplemented by immersing the section in OsteoCal Mild Blue for 15-20 minutes, followed by rinsing with tap water.
RESULTS
Decalcified tissue will resemble cartilage, making it suitable for further histological procedures.
NOTE
Prolonged decalcification may negatively impact tissue
morphology and limit subsequent nucleus staining. For
immunohistological methods requiring intact blood antigens, consider using OsteoCal Yellow.
USABILITY
30 ml of OsteoCal Mild Blue, enough to cover the entire
section, is sufficient for 2 uses. Ensure the solution
remains clear and uncontaminated.
SAMPLE PREPARATION AND DIAGNOSTICS
Follow proper sample collection and preparation techniques, use modern technology, and mark samples clearly. Only authorized and qualified personnel should conduct staining and diagnostics.
SAFETY AND ENVIRONMENTAL PROTECTION
Handle the product following safety and environmental
protection guidelines. Dispose of used and expired solutions as special waste according to national guidelines. Adhere to safety measures for protecting human health.
STORING AND EXPIRY
Store OsteoCal Mild Blue in its original packaging at
temperatures between +15°C to +25°C, avoiding freezing and direct sunlight. Refer to the product label for the date of manufacture and expiry date.
