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The cytological staining technique developed by Papanicolaou is still the standard method in cancer and hormone cycle diagnosis. The effect of the orange staining solution is considerable in smears with keratinized cells under acidic pH conditions, when the obvious orangeophilic cytoplasmic stain is recognizable and may point to the presence of hyperkeratosis, HPV infection or carcinoma cells. Papanicolaou's solution Orange G solution gives a gentle yellow-orange staining result with mature and keratinized squamous cells.