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Sperm Vitality Kit

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Quantitative eosin staining kit for the percentage measurement of live spermatozoa in semen samples GENERAL INFORMATION Sperm vitality is reflected in the proportion of spermatozoa that are “alive”. Sperm vitality should be determined in semen samples with less than about 40% progressive motile spermatozoa. Sperm Vitality Test uses the eosin staining technique to establishes the percentage of live spermatozoa. The technique is based on the principle that dead cells will take up the eosin, and as a result stain red. Sperm Vitality Test provides an accuracy check of the motility evaluation since the percentage dead spermatozoa should not exceed the percentage immotile spermatozoa. Sperm Vitality Test may help in assessing the diagnosis and the management of male infertility. MATERIAL INCLUDED IN THE KIT Reagent A - 1 ml of red stain MATERIAL NOT INCLUDED IN THE KIT Light microscope (400 - 600x magnification) Microscope glasses Cover glasses Pipettes Test tubes (sterile) Microscope slides Laboratory counter METHOD 1. Mix the semen sample well 2. Remove an aliquot of 5 µL of semen and combine with 5 µL of reagent A on a microscope slide. Mix with a pipette tip, swirling the sample on the slide. 3. Cover with a 22 mm x 22 mm coverslip and leave for 30 seconds. 4. Remix the semen sample, remove a replicate aliquot, mix with reagent A and treat as in steps 2 and 3 above. 5. Examine each slide, preferably with negative-phase-contrast optics at x200 or x400 magnification. 6. Tally the number of stained (dead) and unstained (vital) cells with the aid of a laboratory counter. 7. Evaluate 200 spermatozoa in each replicate, in order to achieve an acceptably low sampling error. 8. Calculate the average and difference of the two percentages of vital cells from the replicate preparations. 9. Determine the acceptability of the difference. 10. If the difference between the percentages is acceptable, report the average percentage vitality. If the difference is too high, make 2 new preparations from 2 new aliquots of semen and repeat the assessment. 11. Report the average percentage of vital spermatozoa to the nearest whole number. INTERPRETATION - Colourless spermatozoa: live spermatozoa - Red stained spermatozoa: dead spermatozoa Count between 100 and 200 cells and differentiate the living from the dead spermatozoa. Read results immediately, waiting too long will yield lower vitality percentages. It is clinically important to know whether immotile spermatozoa are alive or dead. Vitality results should be assessed in conjunction with motility results from the same semen sample. The presence of a large proportion of vital but immotile cells may be indicative of structural defects in the flagellum; a high percentage of immotile and non-viable cells (necrozoospermia) may indicate epididymal pathology. A semen sample is considered normal if 58% or more of the sperm cells are alive. LIMITATIONS OF THE METHOD Spermatozoa stained with Renata Sperm Vitality Test cannot be used for any further procedures. STORAGE Suitable for transport or short term storage at elevated temperatures (up to 5 days at 37°C). Store reagent between 2°C and 25°C. WARNINGS AND PRECAUTIONS All human, organic material should be considered potentially infectious. Handle all specimens as if capable of transmitting HIV or hepatitis. Always wear protective clothing when handling specimens. Sperm Vitality IFU ENG PDF

FFA Direct

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Quantitative measurement of Acidity in fats & oils Description The acidity level of edible oils and fats is determined by the amount of free fatty acids produced by the breakdown of triglycerides during hydrolytic rancidity. Since this change takes place under unfavourable conditions for the processing and preservation of fats, acidity serves as a fundamental measure of the authenticity of the product. This test is particularly critical in the refining of oils and fats, as it contributes to the evaluation of the processing cycle and the classification of products. Although it has comparable accuracy, this test is simpler to perform than the official AOCS Ca 5a-40 method. In samples with a pH below 7,0, the free fatty acids present react with a colour-producing compound, resulting in a reduction of the colour intensity. The extent of this reduction in colour is directly proportional to the concentration of acid in the sample, expressed as a percentage of oleic acid. Package contents 1 x 0.75 ml R1, 1 x 0.25 ml R2, 5 x 20 ml R3 Number of tests: 100 tests Ref.: 89491 Shelf life: 24 months from date of manufacture Storage & Stability: 2-8°C Sample collection instructions No preparation of olive oil sample preparation is required. For the analysis of samples such as solid fats, butter, margarine, cream, nuts, flours and other extracted fatty foods, it is recommended to follow the guidelines described in the enclosed 'Preparation of test sample for analysis of fatty foods'. This document provides guidance on how to properly prepare these specific samples for analysis and can be obtained from your supplier or from the manufacturer - MenidiMedica Biotech Greece. It will likely include information on techniques such as milling, homogenisation, extraction or other relevant procedures to ensure accurate and representative analysis of the fat content of these foods. Preparation of reagents Preparation WR: Add 150 uL. R1 and 50uL. R2 to one of 5 vials of 20 mL. R3. Screw on the cap of vial R3. The WR changes color to violet and is stable until the expiration date indicated on the outer label of the kit. Procedure - Wavelength: 546 nm - Blank: Air - Method: End Point - WR: 1 mL. - Sample: 20 uL. - Mix and incubate for 60'' (seconds) - Read results Reference values Linearity (for olive oil): 0,01-1,10 (expressed as % oleic acid) For other types of food, please inform the producer MenidiMedica Biotech Greece to provide you with the corresponding protocol with the following linearity towards this type of food. Note: For samples where the analyzer gives you an UPPER LIMIT value, then you should dilute your sample with OLIVE OIL DILUTOR (Ref. 89377, sold separately by MenidiMedica Biotech Greece) in a ratio of 1:1 or 1:5 and multiply the result given by the analyzer by 1 or 5. Security measures The ingredients of FFA-Acidity in fats & oils pose no health risk when used in accordance with standard laboratory practices and the procedures in this insert. For further safety instructions, refer to the Safety Data Sheet (SDS). FFA Direct Quantitative method in biological, food, environmental samples IFU ENG PDF

Histanol 100

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Rehydrating/dehydrating agent of tissue and cytological samples Introduction Histology, cytology, and related scientific fields focus on examining the microscopic structure of tissues and cells. Achieving clear visualization of tissue and cellular structures requires precise sample processing. The histological sample processing involves several key steps, with three involving dehydration and subsequent rehydration. The initial step involves preparing the samples for infiltration, embedding them in paraffin, and then cutting the paraffin blocks into thin slices. In the second step, the samples are prepared for staining, and the final step involves mounting the samples on glass slides. Since most embedding media, like commonly used paraffin, do not readily penetrate samples containing water, it is essential to perform dehydration first to facilitate the infiltration process. Once the samples are embedded in paraffin, cut into thin slices, and mounted on glass slides, they maintain their integrity for a specific period. However, before staining, it is necessary to remove the paraffin and rehydrate the sections. Only then can histological dyes be applied for staining. A similar procedure is followed for cytological samples, with dehydrating agents, primarily consisting of alcohols. One widely used dehydrating agent is denatured ethanol, which serves as the primary component in MenidiMedica Biotech Histanol. Histanol is a transparent, colorless, and flammable liquid known for its rapid action and high efficiency. Product description Other slides and reagents that may be used in staining: - Fixatives such as MenidiMedica Biotech neutral buffered formaldehyde solutions (Formaldehyde NB 10%) - Dehydrating/rehydrating agent, such as MenidiMedica Biotech alcohol solutions - Clearing agents, such as xylene or a substitute agent on the aliphatic hydrocarbons basis - Infiltration and fitting agent, such as granulated paraffin - High-quality glass slides for use in histopathology and cytology - Differentiation agent, such as MenidiMedica Biotech Acid alcohol - Bluing agents, such as MenidiMedica Biotech Scott's solution or Bluing reagent - Covering agents for microscopic sections and mounting cover glass, such as MenidiMedica Biotech Eukitt - Cover glass, dimensions range from 18x18mm to 24x60mm - Reagent for nuclear staining, such as MenidiMedica Biotech Hematoxylin Harris - Counterstaining reagents, such as MenidiMedica Biotech eosin solutions Preparing histological sections for staining - Fix the tissue sample tightly (10% NB Formaldehyde), rinse with water and dehydrate through series of ascending alcohol solutions (Histanol 100). - Clear the sample with intermedium; in xylene or in a xylene substitute. - Infiltrate and fit the sample in paraffin - Cut the paraffin block to 4-6 µm slices and place them on a glass slide Hematoxylin and eosin (HE) staining procedure, progressive - Deparaffinize the section in xylene or in a xylene substitute - 3 exchanges, 2 min each - Rehydrate using 100% alcohol (Histanol 100) - 2 exchanges, 5 and 3 min - Rehydrate using 95% alcohol - 2 min - Rehydrate in distilled water - 2 min - Stain using Hematoxylin Harris - 3-5 minutes Note: In the case of subsidence in the solution or a formation of metallic glow on the surface, reagent should be filtrated before use. - Immerse the section in distilled or demineralized water until dye is no longer being released from the section - Make nuclei turn blue using Scott's solution or Bluing reagent - 1 min Note: Finish the process of bluing after the nuclei turn blue If no Scott's solution or Bluing reagent is available, rinse the sections under tap water for 3-5 minutes. - Stain with one of eosin contrast solutions until the section is optimally stained - 15 seconds - 2 minutes Note: Staining the sections in eosin alcoholic solutions causes intensive eosinophil color to show much faster (in under 15 seconds' time). Recommended exposure time for eosin aqueous solutions is 90 seconds to 2 minutes - Rinse under tap water - 2 min - Dehydrate using 95% alcohol - 2 exchanges, 10-15 dips - Dehydrate using 100% alcohol (Histanol 100) - 3 exchanges, 10-15 dips - Clear the section in xylene or in a xylene substitute Immediately after clearing apply an appropriate mount medium for covering/mounting on the section. If xylene was used, use one of mounting xylene-based media. If xylene substitute was used, use the appropriate covering agent for this case. Cover the section with a cover glass. Result Nucleus - dark blue Cytoplasm, collagen, elastin, erythrocytes - various shades of pink (when staining with Eosin Contrast the shade is red-pink) Note Time periods of staining processes are not entirely standardized and they approximately correspond to clinical and laboratory practical experience. Intensity of staining depends on the period of immersion in the dye. Real staining protocol depends on personal requests and priorities. Preparing the sample and diagnostics Use only appropriate instruments for collecting and preparing the samples. Process the samples with modern technology and mark them clearly. Follow the manufacturer's instructions for handling. In order to avoid mistakes, the staining procedure and diagnostics should only be conducted by authorized and qualified personnel. Use only microscope according to standards of the medical diagnostic laboratory. Safety at work and environmental protection Handle the product in accordance with safety at work and environmental protection guidelines. Used solutions and out of date solutions should be disposed of as special waste in accordance with national guidelines. Chemicals used in this procedure could pose danger to human health. Tested tissue specimens are potentially infectious. Necessary safety measures for protecting human health should be taken in accordance with good laboratory practice. Act in accordance with signs and warnings notices printed on the product's label, as well as in MenidiMedica Biotech material safety data sheet. Storing, stability and expiry date Keep Histanol in a tightly closed original package at temperature between +15°C and +25°C. Keep in dry places, do not freeze and avoid exposing to direct sunlight. Date of manufacture and expiry date are Histanol 100 IFU ENG PDFprinted on the product's label.

Acetic Acid 5% & 8%

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Content Acetic Acid, Preservative Description Acetic Acid How Supplied Glass bottles of 100, 200, 500, 1000 mL. Action & Uses Acetic acid is an agent used for differentiating normal from suspicious tissues. Normal tissue remain as they are, while tissue suspicious for mmalformations develops areas of white. Wash after use with water or normal saline. Warnings For External Use Only. Should inadvertent ocular administration occur, the eye(s) should be washed immediately with large amounts of water or normal saline, occasionally lifting the upper and lower lids until no evidence of solution remains (approximately 15-20 minutes). Storage Keep tightly closed and protect from light. DO NOT use if seal is broken. Store at room temperature 15°- 30°C. Disposal Opened containers with unused portions of product should be placed in a suitable, dry container for later disposal according to local hazardous waste practices Acetic Acid 5% & 8% IFU ENG PDF

Peroxide Value in Fats & Oil

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Quantitative detection of Peroxide Value (P.V.) in fats and oil Our kit method detects the determination of peroxide values for animal oils and fats, vegetable oils and fats, as well as for flavour and fragrance materials. The peroxide value is a parameter specifying the content of oxygen as peroxide, especially hydro peroxides in a substance. The peroxide value is a measure of the oxidation present. MenidiMedica Biotech's method shows a very good correlation with AOCS Official Method Cd 8-53. Furthermore, P.V. was determined using Electra m2 Unified Analyzer and the UKAS accredited titration method used by Campden BRI (Campden BRI Method TES-AC-360). The data for P.V. using Electra m2 Unified Analyzer was found to be very consistent with good reproducibility across the triplicate runs and the correlation between the two methods is good. Presentation Ref.No: 89010 - 50 tests/kit, 24 months at 2-30°C 5 x R1 ---> 0.5 mL. with distilled water R2A - 1.25 mL. R2B - 1.25 mL. R3 - 0.05 mL. R4 - 490 mL. Reagent Preparation R1: Each vial of lyophylized R1 is adequate for 10 exams. Reconstitute R1 with 500 uL. distilled water. The solution is stable for 30 days. WR2: Mix equal quantities of R2A and R2B with R3 at a ratio 50:1, mix gently. Centrifuge the solution at 3000 rpm for 3 minutes and collect the supernatant. Label it as WR2, the solution is stable for 10 days at RT. R3: Ready to use, Handle with caution, use gloves R4: Ready to use *Note: R2A and R2B are photosensitive, store in a dark place. Test procedure Mix 9.8 mL. of reagent R4 with 0.1 gr. of sample, mix gently for 3 seconds. Add 50 uL. R1 and mix gently for 3 seconds. Then, add 50 uL. of WR2 and mix gently for 3 seconds. Incubate 5 miutes at RT and read results in Electra m2 Unified Analyzer choosing the parameter P.V. in Foods. The kit is stable for 2 years from the production date. Store it at RT away from direct sunlight. The assay is compatible with all the available commercial spectrophotometers. For instructions, contact MenidiMedica Biotech or an authorized representative. SAMPLE PREPARATION FAT OILS

Condylen Forte

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Contains TCA, Podophyllum Resin, Preservatives Description Condylen Forte How Supplied 20 gm glass bottles Actions & Uses Condylen Forte is a product employed to remove external warts. The recommended application method involves applying the liquid to the warts at specified intervals, which should be every 15 days, and repeating this process twice. After use, it is important to rinse the treated area with either water or a standard saline solution. 1. The physician identifies the warts in the affected area. 2. Using a pointed applicator tip, apply a sufficient amount of the solution directly to the top of each wart. Be careful not to apply it to the surrounding healthy tissues because the solution has a cauterizing effect on warts. 3. Following the initial application, the patient should use protection during sexual intercourse only on the first day of application. 4. Reapply the solution on the 16th day. 5. On the 31st day, the patient should undergo a Pap Test and an HPV test. Warnings For External Use Only. Should inadvertent ocular administration occur, the eye(s) should be washed immediately with large amounts of water or normal saline, occasionally lifting the upper and lower lids until no evidence of solution remains (approximately 15-20 minutes). Storage Keep tightly closed and protect from light. DO NOT use if seal is broken. Store at room temperature. Disposal Opened containers with unused portions of product containing residual product should be placed in a suitable, dry container for later disposal according to local hazardous waste practices. Condylen Forte IFU ENG PDF

Qualis Oleum

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RAPID TEST DETECTING TOTAL POLAR COMPOUNDS IN FRYING FATS AND OILS Qualis Oleum is a quick test for the semi-quantitative detection of TPC (Total Polar Compounds) in frying fats. Ideal for everyday quality control of vegetable oils (sunflower oil, corn, peanut, etc.). It is an excellent solution for fast foods, restaurants, hotels, supermarkets, caterings, food industries, etc. Acrylamide does not naturally occur in cooking oil, but when starchy foods such as potatoes are fried with re-used oil, then acrylamide levels can reach dangerously high levels. Increasing the concentration of acrylamide in cooking oil can be particularly harmful to the consumers of the products. It is accepted by the scientific community that high levels of TPCs contain high levels of acrylamide . It is universally accepted that daily monitoring of TPC levels of cooking oil is essential to ensure its quality. CHARACTERISTICS Sample Preheated specimen at 60°C or no preheated specimen at 20°C Contents kit Each kit contains 10 or 50 pre-filled tubes with reagent R. Test procedure 1. Add 500 uL. of sample in the pre-filled tube 2. Mix gently for at least 5 seconds 3. Read the color results. Compare formed colors with color code Interpretation of the results (For quantification, compare to the specific color on the card included in the kit) Deep green: Good oil quality, keep using it Bright yellow: Bad oil quality, change required Reference Code: 82350 Linearity range: 0-25 (in % of TPC concentration) Expiry date: 24 months Storage: The kit can be preserved at room temperature QUALIS OLEUM (1)

KaryoPrep Solution for Fixation

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KaryoPrep Solution for Fixation is the fixative liquid specially dedicated to the preservation of cytological samples. It is designed, developed and produced by MenidiMedica Greece and it can be used with all kinds of biological samples. KaryoPrep Solution for Fixation is designed for use with the manual method introduced by MenidiMedica or with MenidiMedica LBC system or other commercial processors. It serves as a transport, preservative, and antibacterial medium for the processed biological samples, including certain tests for Human Papilloma Virus (HPV) and other sexually transmitted infections. It has no haemolytic activity in order to ensure conservation of red blood cells, eventually present in urinary samples, to make them visible and diagnosable by the pathologist during the specimen analysis. In case the user wishes elimination of mucus or red blood cells (absence of artifacts), KaryoPrep RBC Lytic Reagent must be applied to the KaryoPrep Solution for Fixation. KaryoPrep Solution for Fixation is a saline solution based on ethanol, which ensures the perfect cytological material fixation and a safe operator's handling at the same time. It guarantees the morphological preservation of cytological samples for at least 5 years at room temperature. The genetic material of the collected samples is stored and available for molecular biology in-depth analysis, up until 5 years after collection. For optimal samples fixation, it is recommended to use unexpired vials of KaryoPrep Solution for Fixation. The vials are ready-to-use and are stable for 60 months at room temperature. Karyoprep Solution for fixation is supplied in different packaging, ranging from 10 mL. prefilled collection vials, to 1 or 5 Lt. - bottles approved for air transport. The vials are made of a special transparent plastic material, characterized by peculiar optical transmittance technical specifications, allowing them to be used with the MenidiMedica LBC fitting system and with various other platform systems. The 1 Lt - formats are supplied in HDPE plastic material bottles. HDPE material, certified by the provider, is free of BPA, phthalates and latex and does not contain or contains less than the 0.1|% of substances that can be defined as ''high concern'', identified as SVHC and listed in the candidates list in the XIV REACH annex. The 5 Lt - formats are provided in stackable plastic tanks, UN/ADR approved for land, air and sea transport of dangerous liquid products, according to UN Legislation. LBC LEAFLET

Total Polyphenols Direct

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Quantitative Determination of total polyphenols in edible oils, wine and food Description The antioxidant capacity of polyphenols plays an important role in the stability of olive oil, as there is a correlation between the amount of total polyphenols and resistance to oxidation. Polyphenols have a protective effect on the cells of the human body because they oppose the negative effects of free radicals. These polyphenols in olive oil act as natural antioxidants. The amount of polyphenols decreases during olive oil extraction, so the test can be used to optimize the processing. Olive oil is rich in polyphenols, which form its "polar fraction" and prevent its self-oxidation, thus giving it its excellent thermal stability and contributing to its characteristic aroma and taste. The main ones are: tyrosol, hydroxytyrosol, oleuropein and protocatechuic, gallic, vanillic, vanillic, p-hydroxybenzoic, syringic, 4-hydroxyphenyl acetate, omavanillic, quinamic, o-coumaric, p-coumaric, caffeic, ferulic and sinoacetic acids. The main antioxidants of olive oil are considered to be tocopherols, the main representative being alpha-tocopherol, and phenolic compounds. Vitamin E with its parent compound tocol consists of two series of compounds: tocopherols and tocotrienols. The tocopherols are divided into a-tocopherol, b-tocopherol, c-tocopherol, and d-tocopherol, while the tocotrienols are divided into a-, b-, c-, and d-tocotrienol. The total biological activity of vitamin E is mainly due to the presence of αtocopherol. It is the alpha-tocopherol that exerts the greatest biological activity in the human body and is the reference for the action of the other forms of vitamin E. All tocopherols are natural antioxidants of oils, since they have an antioxidant activity that increases from a to d. The stability of olive oil to oxidation is largely due to the presence of tocopherols, which are easily oxidised. A-tocopherol is traditionally considered the main antioxidant in olive oil. It constitutes about 90% of all tocopherols and normally ranges from 100 to 300 ppm (Blekas et al., 1995; Psomiadou and Tsimidou, 1998). c-Tocopherol constitutes about 8% of all tocopherols in olive oil, while b-, d-tocopherols are only present in trace amounts (Andrikopoulos et al., 1989). Vitamin E is an important natural antioxidant of oils, since it inhibits the oxidation of their fatty substances (triglycerides), and protects olive oil from peroxidation and the free radical propagation mechanism. Package contents: 1 x 5 ml R1а, 3 x 17 ml R1b, 1 x 7.5 ml R2 Number of tests: 50 tests Ref.: 89001 Shelf life: 24 months from date of manufacture Storage & Stability: 2-8°C Sample collection instructions No preparation of olive oil sample is required. Preparation of reagents *WR1: Mix 100 μl R1a with 1000 μl R1b and shake gently for 3 seconds R1а - Mixing with R1b is required R1b - Mixing with R1a is required R2 - Ready for use Procedure - Wavelength: 700 nm on the Electra m2 Unified Analyzer - Temperature: 30°C (25°C – 37°C) - Method: FIXED TIME - Fitting: LINEAR - Select the T.PPS parameter from the test menu - ZERO: WR1 500 uL. + 10 uL. deionized water, Press OK. - ADD REAGENT R1 : WR1: 500 uL. Press OK. - ADD SAMPLE: 10 uL.Press OK. - Wait 180 seconds. - Measurement of absorbance A1. ADD REAGENT R2: 150 uL. Press OK. - Wait 300 seconds. - Absorbance measurement A2. DA = A2-A1 (total polyphenol concentration expressed in mg/l.) Note: A 5-point calibration of 0, 125, 250, 500, 1000 (mg/l) is recommended. Reference values Linearity: 0-1000 mg/l. Note: For samples that the analyzer gives you an UPPER LIMIT value, then you should dilute your sample with OLIVE OIL DILUTOR (Ref. 89377, sold separately by MenidiMedica Biotech Greece) in a ratio of 1:1 or 1:5 and multiply the result given by the analyzer by 1 or 5. Safety measures The components of elean morian polyphenols pose no health risk when used in accordance with standard laboratory practices and the procedures in this insert. For further safety instructions, refer to the Safety Data Sheet (SDS). Notes A 4-level calibration kit is available by MenidiMedica Biotech Greece (Recommended for calibration on automatic instruments). Total Polyphenols Direct Quantitative method in fats, oils, bakery products, nuts, dried fruits IFU ENG PDF

KaryoPrep Fixative

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KaryoPrep Fixative is the protective alcoholic solution designed to protect cells from air contact during the drying phase of the slides prepared with MenidiMedica cytology processors, or with the manual method. Compatible with the reagents of other liquid based cytology technology methods, it can be implemented, as a universal fixative, into the sample processing of competition. It is supplied in various volumes in HDPE plastic material. The HDPE material, certified by the provider, is free of BPA, phthalates and latex and does not contain or contains less than the 0.1% of substances that can be defined as ''high concern'', identified as SVHC and listed in the candidates list in the XIV REACH annex. LBC LEAFLET

L-Ascorbic Acid Direct (Vitamin C)

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QUANTITATIVE DETECTION OF L-ASCORBIC ACID Ascorbic acid is a nutrient the human body needs in small amounts to function, and it can help prevent cell damage caused by free radicals— unstable molecules that can damage cells. It can also help the human body fight bacterial infections. Cosmetics and other personal care products may include less acidic forms of ascorbic acid, which can act as antioxidants to slow product deterioration. The FDA states that ascorbic acid is a generally recognized as safe substance for use as a chemical preservative in foods and as a nutrient or dietary supplement. The Cosmetic Ingredient Review states that ascorbic acid and its salts are safe for use in cosmetic and personal care products. According to the U.S. National Cancer Institute, ascorbic acid can help the human body fight bacterial infections and help form collagen, an important protein in fibrous tissue, teeth, bones, skin and capillaries. CHARACTERISTICS Reference: 82905 Linearity range: 0.2-50 mg/dL. L-ascorbic acid content Presentation: Chromogen Activator R1: 20 mL., Substrate R2: 20 mL. Sample Matrices: food, fruits, cosmetics, juices, pharmaceuticals Expiry Date: 24 months *The kit must be stored at 2°-8°C HOW TO USE Method: Quantitative, Endpoint Wavelength: 545 nm Blank: Water Procedure: 1. Read Blank 2. Transfer 200 uL. R1 into a cuvette 3. Add 200 uL. R2 into the cuvette 4. Add 50 uL. sample into the cuvette and mix incubate for 30'' 6. Read results The color is stable for 40' L-ASCORBIC ACID DIRECT ENG PDF

Lactopast Biomedix Fast Phosphatase

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Instructions for use We have researched and developed a fast qualitative test of milk pasteurization with no need of any instrumental or incubational means with the name LACTOPAST BIOMEDIX. It is a rapid phosphatase test for the detection of alcaline phosphatase in milk, whey, cream and butter. The test allows the testing of sufficient HTST treatment of milk and related products, and the detection of raw milk in pasteurized milk. Sensitivity of the test is 3.5 mU/L.

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