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Ref. Number: 70996
Ref.No.: 70997
Presentation: 75300-10:10mL. 75300-20:20mL. 75300-50:50mL. 75300-100:100mL. Description: - E read more →
Ref.No.: FX100025 - 25 vials, FX100100 - 100 vials



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MenidiMedica

Medical Instruments, Tools & Supplies in Etoloakarnania in Greece.
Our highly experienced and committed team focus all their energy to deliver what our customers demand at very competitive prices, ensuring your business success.

Postal address
Menidi Aetolias & Akarnanias
Zip code: 30016, Greece

Customer Support

T +30 2681088000
Μ +30 6937115868
Μ +30 6976292146
menidimedica@gmail.com

Business Hours
From Monday through Friday
08:00 – 17:00 (EET)

INFORMATION
MenidiMedica © 2009 – 2025. All Rights Reserved.

OSTEOCAL MILD BLUE

LIGHT BLUE FIXATIVE AND DECALCIFYING SOLUTION - IDEAL FOR BONE AND HARD TISSUE IN HISTOLOGY INTRODUCTION In the field of histology, the microscopic analysis of bone and hard tissue samples is essential for medical research and diagnosis. To achieve accurate results, it is crucial to conduct decalcification of these samples. OsteoCal Mild Blue is the solution you need for efficient and precise decalcification. THE DECALCIFICATION PROCESS Decalcification is the process of removing calcium from bone and hard tissue, allowing for further microscopic analysis. The duration of decalcification varies based on the sample's size and density. OsteoCal Mild Blue, containing inorganic hydrochloric acid, rapidly softens the tissue and prepares it for analysis. This solution is suitable for bone, teeth, and keratinized tissue samples such as filiform warts and nails. PRODUCT DESCRIPTION - OSTEOCAL MILD BLUE: Light blue fixative and decalcifying solution for bone and hard tissue in histology. Contains formaldehyde and hydrochloric acid. - Other Compatible Reagents: Explore our range of complementary products, including fixatives, dehydrating agents, clearing agents, infiltration agents, immersion media, staining reagents, and more. PREPARING THE SAMPLE FOR DECALCIFICATION 1. Fixation: Ensure the tissue sample is properly fixated. 2. Immerse: Place the tissue sample into OsteoCal Mild Blue for complete decalcification. DECALCIFICATION GUIDELINES Bone, Teeth, Hard Tissue: Duration: 6-8 hours for a 1 x 1 x 0.3 cm bone (e.g., femur). Mildly Calcified Tissue (e.g., blood vessels): Duration: 30-60 minutes. Keratinized Tissue (e.g., nails, filiform warts): Duration: 15-60 minutes with the cross-section oriented downward. ENSURING COMPLETION Use a needle to puncture an unimportant part of the sample to determine the end of the decalcification process. Incomplete decalcification can be supplemented by immersing the section in OsteoCal Mild Blue for 15-20 minutes, followed by rinsing with tap water. RESULTS Decalcified tissue will resemble cartilage, making it suitable for further histological procedures. NOTE Prolonged decalcification may negatively impact tissue morphology and limit subsequent nucleus staining. For immunohistological methods requiring intact blood antigens, consider using OsteoCal Yellow. USABILITY 30 ml of OsteoCal Mild Blue, enough to cover the entire section, is sufficient for 2 uses. Ensure the solution remains clear and uncontaminated. SAMPLE PREPARATION AND DIAGNOSTICS Follow proper sample collection and preparation techniques, use modern technology, and mark samples clearly. Only authorized and qualified personnel should conduct staining and diagnostics. SAFETY AND ENVIRONMENTAL PROTECTION Handle the product following safety and environmental protection guidelines. Dispose of used and expired solutions as special waste according to national guidelines. Adhere to safety measures for protecting human health. STORING AND EXPIRY Store OsteoCal Mild Blue in its original packaging at temperatures between +15°C to +25°C, avoiding freezing and direct sunlight. Refer to the product label for the date of manufacture and expiry date. Osteocal Mild Blue ENG PDF

TETHYS: UNIQUE RAPID CARIES DETECTION TEST

THE FUTURE OF ORAL HEALTH: UNIQUE RAPID CARIES DETECTION TEST JOIN THE ‘BETTER ORAL HEALTH MOVEMENT FOR ALL’ The majority of participants in our clinical trial (90%) recognised the importance of early detection of caries. Boost your oral health and enjoy a healthier smile. TAKE CHARGE OF YOUR ORAL HEALTH Globally, in 2019, 3.09 billion new cases of caries in permanent teeth were recorded (an increase of 48.00%), as well as 2.03 billion existing cases of caries (an increase of 46.07%), compared to 1990. Among adolescents aged 12 to 19 years, more than half (57%) had at least some dental decay from caries in their permanent teeth. In adults aged 20 years and older, 90% had at least one dental decay from dental caries. EARLY DETECTION FOR A HEALTHIER SMILE Tooth decay is a silent threat to your oral health, but with our revolutionary TETHYS rapid caries detection test, you can now detect it early and take control of your dental wellbeing. WHY CHOOSE THE RAPID CARIES DETECTION TEST? Product of cooperation between MenidiMedica Biotech Greece and the University of West Attica. TETHYS diagnostic test for valid, early detection of dental caries, ensuring oral hygiene. - Fast and reliable: you get results within minutes, allowing early detection of caries even in its early stages. - Accurate: Our test has high sensitivity and specificity, ensuring accurate detection of caries. - Convenient: No invasive procedures or specialized equipment are required. A simple swab and a vial is all that is needed. - Safe: The test is safe and non-invasive, making it suitable for all ages. - Proven results: Backed by clinical trials, our test has shown excellent performance. HOW IT WORKS? - Open the sterile wooden swab. - Brush your teeth as usual. - Open the vial of single reagent and rinse the head of the swab in the vial. - Break off the wooden swab head and place it in the vial - Close the vial and shake gently for 5 seconds. - Place the vial in your pocket for 30 minutes. - Check for a colour change in the reagent: a. Magenta for a positive result. Have caries in progress b. Pink or transparent clear for a positive result. You have caries and a decaying tooth c. Purple for a negative result. You do not have caries DETECT-PREVENT-RESIST Don't let tooth decay develop into a painful problem. With the TETHYS quick caries test, you take control of your oral health in your own hands. CLINICAL TRIAL RESULTS - Sensitivity: 98,39% - Specificity: 100.00% - Positive predictive value (PPV): 100.00% - Negative predictive value (NPV): 99.19% - Accuracy: 99.46% tethys eng pdf

VagoClean

Your Solution for Persistent Bacterial Vaginal Infections. - Reagent in 10 mL. Single Dose in packages of 5x10 mL. and 10x10 mL. - Ref. Number: 70996-5, 70996-10 “Experience Relief and Well-Being” What is a Bacterial Vaginal Infection? Bacterial vaginal infections are common health issues affecting women of all ages. They occur when the delicate balance of bacteria in the vaginal area is disrupted,leading to discomfort and unpleasant symptoms. Such infections are typically caused by an overgrowth of harmful bacteria, and they can result in: - Itching and Irritation - Abnormal Vaginal Discharge - Burning Sensation - Unpleasant Odor If left untreated, bacterial vaginal infections can lead to more serious complications, including: - Pelvic Inflammatory Disease (PID) - Recurrent Infections - Premature Birth (in pregnant women) “Introducing VagoClean - Your Solution!” Easy-to-Use Kit VagoClean, a product of MenidiMedica Biotech Greece, is here to help you take control of your vaginal health. Our 10 mL single-dose reagent offers a convenient and effective solution for cases where traditional therapeutic treatments have not yielded the desired results. How It Works 1. Immerse the content of the monoreagent in sterile gauze provided. 2. Gently insert the sterile gauze into the vagina for local washing. 3. Leave it in for 1 minute. 4. Remove the sterile gauze. If symptoms of bacterial infection persist, repeat the medical procedure after 8 calendar days with VagoClean for comprehensive relief. Benefits of VagoClean - Fast and Effective Relief - Maintains Vaginal Balance - Easy-to-Use Kit - Sterile and Safe - Supports Your Well-Being VagoClean is intended for use by gynecologists only. It is a specialized product designed to address bacterial vaginal infections in a clinical setting under the guidance of healthcare professionals. Please consult with your gynecologist for proper usage and treatment. VagoClean Commercial Leaflet ENG pdf

VAGOHEALTH

INTRODUCING VAGOHEALTH: THE ULTIMATE SOLUTION FOR TRICHOMONIASIS Are you aware of Trichomoniasis? It's time to take control of your intimate health with VagoHealth – the revolutionary plant-based solution for the selective treatment of Trichomoniasis, a common sexually transmitted disease (STI). Don't let Trichomoniasis disrupt your intimate life; choose VagoHealth for a healthier, happier you! WHAT IS TRICHOMONAS VAGINALIS? Trichomonas Vaginalis is a parasitic protozoan responsible for Trichomoniasis, one of the most prevalent STIs globally. This microscopic parasite can affect both men and women, leading to various complications if left untreated. THE IMPACT ON MEN AND WOMEN: Trichomoniasis can cause severe health issues for both genders, including: For Women: - Grey or yellowish vaginal discharge - Vaginal bleeding - Genital burning and swelling - Frequent urination - Pain during intercourse - Increased risk of complications during pregnancy, such as premature delivery and low birth weight - Potential female sterilization For Men: - Urethral discharge - Burning during urination or after ejaculation - Frequent urination - Risk of male sterilization MAINTAIN YOUR VAGINAL PH BALANCE A healthy vaginal pH level should ideally be between 3.8 and 4.5, similar to the pH of a tomato. Many women unknowingly disrupt this balance by using soaps for vaginal cleaning, leading to a host of issues. THE IMPORTANCE OF VAGINAL FLORA A balanced vaginal flora dominated by acidophilic lactobacillus and bifidus lactobacillus can protect against infections of the reproductive system. These beneficial bacteria act as a defense against harmful invaders like Neisseria Gonorrhea, Gardnerella Vaginalis, and Trichomonas Vaginalis. Over 50% of women with bacterial vaginosis experience recurrences. DON'T LET TRICHOMONIASIS COMPROMISE YOUR HEALTH! WHY CHOOSE VAGOHEALTH? MenidiMedica Biotech Greece, the Faculty of Public Health at the University of West Attica, and the Laboratory of Molecular Biology and Immunology at the Department of Pharmacy, University of Patras present VagoHealth, a plantbased formulation designed to selectively treat Trichomoniasis within just 48 hours. Our plant based product is laboratory-tested and proven to eliminate Trichomonas Vaginalis without disrupting the natural vaginal flora. VagoHealth offers a revolutionary solution for the selective treatment of Trichomoniasis. Here's why you should consider it: - Swift Elimination: VagoHealth is designed to eradicate Trichomonas Vaginalis within 48 hours, providing rapid relief and peace of mind. - Preserves Vaginal Flora: Unlike some treatments that disrupt the natural vaginal flora, VagoHealth has been meticulously formulated to protect it. It won't harm the beneficial bacteria that help maintain a healthy pH level. - Easy to Use: Our VagoHealth plant-based kit make treatment a breeze. JOIN THE MOVEMENT FOR BETTER VAGINAL HEALTH Did you know that 42% of women claim they were never taught how to take care of their vaginal hygiene? With VagoHealth, you can take control of your intimate health and maintain a balanced vaginal flora to protect against infections. HOW TO USE VAGOHEALTH - Collect a sample from the vaginal or urethral area. - Use the VagoCheck kit for quick identification. - Apply VagoHealth topically as directed by your physician, urologist, or gynecologist in accordance with the prescribed treatment regimen, which may extend up to 48 hours SCIENTIFICALLY PROVEN RESULTS In a laboratory study conducted in collaboration with MenidiMedica Biotech Greece, the Faculty of Public Health at the University of West Attica, and the Laboratory of Molecular Biology and Immunology at the Department of Pharmacy, University of Patras, VagoHealth effectively eliminated Trichomonas Vaginalis within 48 hours, while preserving normal vaginal flora. Results: - 24 Hours: Trichomoniasis cells in the control medium: 1,700,000 cells. With VagoHealth: 30,000 cells. - 48 Hours: Trichomoniasis cells in the control medium: 1,300,000 cells. With VagoHealth: Eliminated! CHOOSE VAGOHEALTH FOR A HEALTHIER YOU! VAGOHEALTH COMMERCIAL LEAFLET ENG PDF

15.5% Ferric Sulfate Solution

Presentation: 75310-10:10mL. 75310-20:20mL. 75310-50:50mL. 75310-100:100mL. Overview: An aqueous based 15.5% Ferric Sulfate solution, which is excellent for soaking retraction cord, eliminating sulcular fluid contamination, aiding gingival retraction and reducing mucous membrane inflammation. 15.5% Ferric Sulfate solution achieves hemostasis in seconds! Description: It is intended for use in arresting various degrees of oral capillary bleeding or sulcular fluids during dental treatment as well as oral surgery—including fixed prosthodontics, restorative operative, and periodontal treatment. 15.5% Ferric Sulfate solution may also be used for retrofillings, tooth impactions, gingivectomies and as a “fixative” for pulpotomies. Indications for Use/Intended Purpose: For use in arresting oral capillary bleeding and/or sulcular fluids in restorative, operative, and surgical dentistry. 15.5% Ferric Sulfate Solution pdf

25% Aluminum Sulfate Solution

Presentation: 75300-10:10mL. 75300-20:20mL. 75300-50:50mL. 75300-100:100mL. Description: - Excellent retraction, hemostatic control and tissue rigidity - 25% Aluminum Sulfate Liquid - Special lubricating vehicle prevents adhesion between retraction cords or impression materials and the gingival tissue - Higher pH, less acidic aluminum chloride solutions - No adverse reaction to impression material 25% Aluminum Sulfate Solution

KASTELANI REAGENT

DESCRIPTION: Kastelani is a combined preparation with the properties of an antiseptic with an antifungal effect. PHARMACOLOGICAL ACTION: Kastelani substances have a wide spectrum of antiseptic and antifungal effects in case of damage to the skin and mucous membranes of the patient by infectious and fungal diseases. FORM OF RELEASE: Kastelani is available as a solution for external use by the patient. The composition of Kastelani includes: phenol, resorcinol, boric acid basic fuchsin. INDICATIONS OF USE: According to the instructions to Kastelani, it is used to treat and prevent skin diseases and their damage. For example, Kastelani's testimony tells us that the reagent is justified in case of abrasions, fungus, ulcers, cracks, surface wounds, pyoderma, erosion in the patient. HOW TO USE: According to the instructions to Kastelani, it must be applied externally. The solution is applied to the affected skin with a cotton swab or a stick 2 to 4 times a day. After drying Kastelani on the skin, you can apply additional medicines in the form of ointments or pastes. SIDE EFFECTS: According to reviews on Kastelani, the reagent can cause various allergic reactions, dermatitis, burning sensation and pain, visual impairment, and also addictive effect, as a result of which Kastelani ceases to have a therapeutic effect on the affected skin. Also, the use of Kastelani is not recommended for a large area of the skin, since it is possible to overdose with phenol, which has the property of actively evaporating from the surface of the skin after application, easily penetrates the blood and can cause dizziness, weakness, breathing disorder, sudden changes in blood pressure. CONTRAINDICATIONS: The use of Kastelani is not possible in cases of hypersensitivity to Kastelani components, detection of a tendency in the patient for increased skin sensitivity, allergic reaction to the drug, chronic dermatoses. LIMITATIONS: Kastelani's instructions prohibit the use of the reagent for pregnant and lactating women. Also, you should be careful when applying Kastelani on the mucous membranes, as it is possible to get chemical burns and irritation. It is also not recommended to assign Kastelani to patients under the age of 12 years. When applying Kastelani simultaneously with other drugs for external use, compounds with unpredictable skin effect can form. KASTELANI REAGENT

Monocyto-ID Listeria Scan

Standard method for biochemical detection and identification of Listeria monocytogenes Description Listeriosis is a serious infection, classified as a zoonosis, which results from the ingestion of food contaminated with the bacterium Listeria monocytogenes. It leads to a severe clinical picture in infants, immunocompromised individuals and poses significant risks to foetuses, while pregnant women usually have mild symptoms. Contents FX100025 - 25 vials R FX100100 - 100 vials R Note: All kit components are stable until the expiration date on the label. Protect them from light and contamination during use. Do not use the reagents after the expiration date. Store reagents at 2-8°C. Shelf life: 12 months from date of production Storage and stability: 2-8°C Samples Food, milk, dairy products, fish Note: for sample preparation, please contact the scientific support department of MenidiMedica Biotech Procedure - Incubation of the sample in suitable nutrient medium (e.g. Ottaviani Agosti Agar Base) for 24 hours at 37⁰C in an incubator - Place a vial - found on the package - with the Listeria monocytogenes biochemical detection and identification reagent in the incubator at the same time as the incubation described in step a is performed. - Remove the medium and the vial from the incubator. - Open the vial and add one drop or 50 ul of deionised water to the vial - Collect a formed colony from the nutrient medium with a swab and dip it into the vial, shaking gently until the contents of the swab are dissolved in the vial. - Incubate the vial in the incubator at 37⁰C for 6 hours - Remove the vial from the incubator and check the colour that has appeared Interpretation Green: positive sample for Listeria monocytogenes Sensitivity: 100% for Listeria monocytogenes Specificity: 100% for Listeria monocytogenes A comparison study with other methods was performed on a total number of samples n=500. Monocyto-ID Listeria Scan PDF ENG

SPERM FERTILITY CHECK

THE ART OF MALE FERTILITY Self Test for Male Fertility Potential General information Around 10-15% of reproductive-age couples experience infertility, and 40-50% of these cases are linked to sperm-related issues. The Sperm Fertility Check is a tool designed to assess whether a sperm sample exhibits normal or subnormal activity. Sperm with normalactivity levels are more likely to improve the likelihood of achieving pregnancy. Warnings and precautions Keep the kit out of reach of children. The blue dye included is toxic and should not be ingested as it poses a risk if swallowed. It can also irritate the skin, eyes, and respiratory tract. Ensure you thoroughly read all the information provided in this pamphlet before conducting the test. Familiarize yourself with the procedure first. To reduce the risk of contaminating the dye, avoid any contact with the tip of the dye bottle. Storage and use The Sperm Fertility Check kit is effective for up to 24 months from the manufacturing date and should be kept within a temperature range of 2-30°C in its original, sealed packaging. Opening the color dye bottle does not affect its shelf life, provided there is no contact with the bottle's tip or the dye itself. Conduct the test at a room temperature between 20-30°C. Performing the test in an environment that is too cold may cause the water's temperature in the cup to drop rapidly, potentially leading to inaccurate, false negative results. The test tubes and funnels are designed for single use only; dispose of them after one use and use a new set for any subsequent tests. Material included with the kit - color chart - funnel x 2 - test tube x 2 - water cup with snap cap - thermometer x 2 - squeeze bottle with dye - Sterile urobox x 2 Material not included with the kit - Hot water (48°C to 50°C), a drinking glass and incandescent light. Instructions for use 1. The reagent bottle's dye should be a dark blue color, matching the "Control" hue on the color chart. If the dye appears clear or pink, it indicates a defect, and you should return the kit. If the dye's color is as expected, proceed to step 2. 2. Open an urobox by removing its packaging. Take off the cap and collect the sample by masturbating directly into the urobox. 3. Allow 30 minutes for the sperm sample to liquefy and filter through the funnel into the test tube. After this period, remove the funnel and dispose of it in the trash together with the urobox. 4. Observe which number on the test tube aligns closest to the top of the sperm sample. If the top of the sample falls midway or more between two numbers, record the higher number. This will determine the number of dye drops you need to add to the sperm sample. If the sperm sample's top is below the number 1, which is etched on the conical part of the test tube, the sample is too small for an accurate test result. Turn the reagent bottle upside down over the test tube and carefully squeeze out the specified number of dye drops onto the sperm sample. Reattach the cap to the test tube and shake it well to mix the dye with the sperm sample. The mixture should now turn blue or purple. 5. Turn on the hot water until it becomes just too hot to comfortably touch. Fill a drinking glass to approximately 3/4 of its capacity with hot water (ensure it's not boiling) and attach the thermometer in the drinking glass. Once the correct temperature 48°C to 50°C is achieved, insert the test tube into the water. Record the time. Avoid placing the drinking glass near open windows or air conditioners to maintain temperature stability. Practicing this step prior to sample collection can be beneficial. 6. Sixty minutes after placing the test tube in the drinking glass, carefully remove it, dry the exterior with a towel or tissue, and then shake the test tube vigorously 4 or 5 times. This action is intended to ensure the dye is thoroughly mixed throughout the sperm sample, achieving even color distribution. 7. Place the white section of the color chart behind the sperm sample to compare its color. Hold both the test tube and the color chart approximately 7 to 10 cm (3 to 4 inches) away from an incandescent light source (avoid using fluorescent lighting). Slide the test tube across the row of colors on the chart to find the closest match, understanding that it might not be an exact match. Observe whether the closest color match falls within the Positive (+) group, indicating normal fertility potential, or the Negative (–) group, suggesting less than normal fertility potential. It's crucial to ensure that no fluorescent lights are on while assessing the test results to avoid any discrepancies in color perception. Interpretation Due to the test's expected color transition from dark blue to red and pink, individuals with red color blindness are advised against interpreting the results. If the color aligns with the Positive group, there is an 86% likelihood of normal sperm activity (20 million or more active sperm per milliliter of fluid), indicating favorable fertility potential. However, this does not assure pregnancy, as various factors influence fertility in both partners. Should pregnancy not be achieved within four months, it's recommended to seek medical advice. Conversely, if the color matches the Negative group, there's an 86% chance that sperm activity is below normal (fewer than 20 million active sperm per milliliter of fluid), making pregnancy less probable, though not impossible. In such cases, consulting a physician is advisable. A Negative result should not be interpreted as an impossibility of pregnancy nor should it be considered a justification for forgoing contraception. Questions and answers - Why does the kit contain material for two tests? The kit includes materials for two tests because sperm quality can fluctuate between samples from the same individual due to various factors such as activity levels, diet, environmental conditions, and other unknown elements. Therefore, the outcome of the first test is best validated by comparing it with a second test conducted after a minimum oneweek interval. Typically, the results of both tests will align. However, if there is a discrepancy between the two, it's advisable to seek a more detailed semen analysis in a medical laboratory to obtain an accurate evaluation. - How long should I abstain prior to the test? Refrain from ejaculating for a minimum of three days and a maximum of ten days prior to conducting the test. - Thirty minutes after ejaculation the majority of the specimen is still not liquefied. Should I wait longer? Yes, wait an additional 10 minutes. If, after this period, the sperm sample has not liquefied the test should be considered invalid and cancelled. It is advisable to consult with a physician in such cases. It is normal for a small amount of the specimen to remain on the urobox, and this should not affect the test's outcome. - The top of my specimen was closest to the number 2. By mistake I added 4 drops of test liquid instead of 2 drops. Should I continue the test? No, the result might not be reliable. Please discard this test and, after waiting three days, conduct another one with the second set of test materials provided. - What can cause a low sperm count or poor sperm movement? Exposure to excessive heat, such as through hot tub use or experiencing fevers, can negatively impact sperm quantity and quality. High fevers can influence test results adversely for up to three months following the illness. The consumption of illicit drugs, excessive alcohol, and smoking cigarettes can all lower sperm counts or impair sperm motility. Furthermore, certain medications can detrimentally affect sperm. Various conditions may lead to reduced sperm quality, but many of these can be treated successfully. If the condition is incurable, artificial reproduction techniques can often still be employed with success in most cases. - Can I collect the sperm sample in a condom? No, typical condoms are treated with a chemical that destroys sperm, making them unsuitable for collecting a sperm sample. However, non-medicated sheaths are availablefor this purpose. Consult your physician regarding where to obtain them. Additionally, it's important not to collect the sperm sample through withdrawal during intercourse, as the initial portion of the ejaculate, which contains the highest quality sperm, may be lost. - My sperm sample did not reach the 1 mark on the test tube. What can I do? Attempt the test again, this time with a longer abstinence period of 10 days. This extended duration may help increase the sperm sample's volume. If, after this second attempt, the sperm sample still does not reach the 1 mark on the test tube, it's advisable to seek further evaluation from a physician. SPERM FERTILITY CHECK ENG PDF

Sperm Vitality Kit

Quantitative eosin staining kit for the percentage measurement of live spermatozoa in semen samples GENERAL INFORMATION Sperm vitality is reflected in the proportion of spermatozoa that are “alive”. Sperm vitality should be determined in semen samples with less than about 40% progressive motile spermatozoa. Sperm Vitality Test uses the eosin staining technique to establishes the percentage of live spermatozoa. The technique is based on the principle that dead cells will take up the eosin, and as a result stain red. Sperm Vitality Test provides an accuracy check of the motility evaluation since the percentage dead spermatozoa should not exceed the percentage immotile spermatozoa. Sperm Vitality Test may help in assessing the diagnosis and the management of male infertility. MATERIAL INCLUDED IN THE KIT Reagent A - 1 ml of red stain MATERIAL NOT INCLUDED IN THE KIT Light microscope (400 - 600x magnification) Microscope glasses Cover glasses Pipettes Test tubes (sterile) Microscope slides Laboratory counter METHOD 1. Mix the semen sample well 2. Remove an aliquot of 5 µL of semen and combine with 5 µL of reagent A on a microscope slide. Mix with a pipette tip, swirling the sample on the slide. 3. Cover with a 22 mm x 22 mm coverslip and leave for 30 seconds. 4. Remix the semen sample, remove a replicate aliquot, mix with reagent A and treat as in steps 2 and 3 above. 5. Examine each slide, preferably with negative-phase-contrast optics at x200 or x400 magnification. 6. Tally the number of stained (dead) and unstained (vital) cells with the aid of a laboratory counter. 7. Evaluate 200 spermatozoa in each replicate, in order to achieve an acceptably low sampling error. 8. Calculate the average and difference of the two percentages of vital cells from the replicate preparations. 9. Determine the acceptability of the difference. 10. If the difference between the percentages is acceptable, report the average percentage vitality. If the difference is too high, make 2 new preparations from 2 new aliquots of semen and repeat the assessment. 11. Report the average percentage of vital spermatozoa to the nearest whole number. INTERPRETATION - Colourless spermatozoa: live spermatozoa - Red stained spermatozoa: dead spermatozoa Count between 100 and 200 cells and differentiate the living from the dead spermatozoa. Read results immediately, waiting too long will yield lower vitality percentages. It is clinically important to know whether immotile spermatozoa are alive or dead. Vitality results should be assessed in conjunction with motility results from the same semen sample. The presence of a large proportion of vital but immotile cells may be indicative of structural defects in the flagellum; a high percentage of immotile and non-viable cells (necrozoospermia) may indicate epididymal pathology. A semen sample is considered normal if 58% or more of the sperm cells are alive. LIMITATIONS OF THE METHOD Spermatozoa stained with Renata Sperm Vitality Test cannot be used for any further procedures. STORAGE Suitable for transport or short term storage at elevated temperatures (up to 5 days at 37°C). Store reagent between 2°C and 25°C. WARNINGS AND PRECAUTIONS All human, organic material should be considered potentially infectious. Handle all specimens as if capable of transmitting HIV or hepatitis. Always wear protective clothing when handling specimens. Sperm Vitality IFU ENG PDF

Acetic Acid 5% & 8%

Content Acetic Acid, Preservative Description Acetic Acid How Supplied Glass bottles of 100, 200, 500, 1000 mL. Action & Uses Acetic acid is an agent used for differentiating normal from suspicious tissues. Normal tissue remain as they are, while tissue suspicious for mmalformations develops areas of white. Wash after use with water or normal saline. Warnings For External Use Only. Should inadvertent ocular administration occur, the eye(s) should be washed immediately with large amounts of water or normal saline, occasionally lifting the upper and lower lids until no evidence of solution remains (approximately 15-20 minutes). Storage Keep tightly closed and protect from light. DO NOT use if seal is broken. Store at room temperature 15°- 30°C. Disposal Opened containers with unused portions of product should be placed in a suitable, dry container for later disposal according to local hazardous waste practices Acetic Acid 5% & 8% IFU ENG PDF

Acid Alcohol 0.5%/1%/3%

Introduction Acid alcohol is a differentiation reagent. It is used in various staining methods, most frequently in regressive hematoxylin eosin (HE) staining and provides excellent differentiation between nuclear and non-nuclear structures. Differentiation rinses dyes from cytoplasm while the nucleus remains stained. That occurs because the nuclear dye bonds stronger to the nucleus than to the cytoplasm. Regardless of the medium the sample is fixated in, Acid alcohol provides satisfying results. Amount of time spent for differentiation using Acid alcohol is always the same regardless of the fixative used for fixating the tested sample. Product description Acid alcohol solution used for differentiation during regressive staining consisting of optimal ratio of hydrochloric acid, ethanol and water. Product use - Acid alcohol is used for section differentiation as a part of regressive staining methods. - Acid alcohol is used for monochromatic and polychromatic staining methods. - One of commonly used staining methods with Acid alcohol is the hematoxylin and eosin method. - Detailed procedure for hematoxylin-eosin staining is described in MenidiMedica Biotech Instructions for use for Hematoxylins used in progressive staining method. Result Acid alcohol is an acidic solution that can be used to differentiate between basic or cationic dyes. Because of higher dye affinity (aluminum-hematein complex) toward nucleus, Acid alcohol will destain cytoplasm. Note Time periods of staining procedures are not standardized. Intensity of staining depends on the period of immersion in the dye. Real staining protocol depends on personal requests and standard laboratory operating procedures. Preparing the sample and diagnostics Use only appropriate instruments for collecting and preparing the samples. Process the samples with modern technology and mark them clearly. Follow the manufacturer's instructions for handling. In order to avoid mistakes, the staining procedure and diagnostics should only be conducted by authorized and qualified personnel. Use only microscope according to standards of the medical diagnostic laboratory. In order to avoid an erroneous result, a positive and negative check is advised before application. Safety at work and environmental protection Handle the product in accordance with safety at work and environmental protection guidelines. Used solutions and out of date solutions should be disposed of as special waste in accordance with national guidelines. Chemicals used in this procedure could pose danger to human health. Tested tissue specimens are potentially infectious. Necessary safety measures for protecting human health should be taken in accordance with good laboratory practice. Act in accordance with signs and warnings notices printed on the product's label, as well as in MenidiMedica Biotech material safety data sheet. Storing, stability and expiry date Keep Acid alcohol in a tightly sealed original packaging at temperature of +15 to +25 °C. Do not keep in cold places, do not freeze and avoid exposing to direct sunlight. Date of manufacture and expiry date are printed on the product's label. Acid Alcohol 0.5%1%3% IFU ENG PDF
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