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KaryoPrep Fixative

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KaryoPrep Fixative is the protective alcoholic solution designed to protect cells from air contact during the drying phase of the slides prepared with MenidiMedica cytology processors, or with the manual method. Compatible with the reagents of other liquid based cytology technology methods, it can be implemented, as a universal fixative, into the sample processing of competition. It is supplied in various volumes in HDPE plastic material. The HDPE material, certified by the provider, is free of BPA, phthalates and latex and does not contain or contains less than the 0.1% of substances that can be defined as ''high concern'', identified as SVHC and listed in the candidates list in the XIV REACH annex. LBC LEAFLET

L-Ascorbic Acid Direct (Vitamin C)

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QUANTITATIVE DETECTION OF L-ASCORBIC ACID Ascorbic acid is a nutrient the human body needs in small amounts to function, and it can help prevent cell damage caused by free radicals— unstable molecules that can damage cells. It can also help the human body fight bacterial infections. Cosmetics and other personal care products may include less acidic forms of ascorbic acid, which can act as antioxidants to slow product deterioration. The FDA states that ascorbic acid is a generally recognized as safe substance for use as a chemical preservative in foods and as a nutrient or dietary supplement. The Cosmetic Ingredient Review states that ascorbic acid and its salts are safe for use in cosmetic and personal care products. According to the U.S. National Cancer Institute, ascorbic acid can help the human body fight bacterial infections and help form collagen, an important protein in fibrous tissue, teeth, bones, skin and capillaries. CHARACTERISTICS Reference: 82905 Linearity range: 0.2-50 mg/dL. L-ascorbic acid content Presentation: Chromogen Activator R1: 20 mL., Substrate R2: 20 mL. Sample Matrices: food, fruits, cosmetics, juices, pharmaceuticals Expiry Date: 24 months *The kit must be stored at 2°-8°C HOW TO USE Method: Quantitative, Endpoint Wavelength: 545 nm Blank: Water Procedure: 1. Read Blank 2. Transfer 200 uL. R1 into a cuvette 3. Add 200 uL. R2 into the cuvette 4. Add 50 uL. sample into the cuvette and mix incubate for 30'' 6. Read results The color is stable for 40' L-ASCORBIC ACID DIRECT ENG PDF

Lactopast Biomedix Fast Phosphatase

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Instructions for use We have researched and developed a fast qualitative test of milk pasteurization with no need of any instrumental or incubational means with the name LACTOPAST BIOMEDIX. It is a rapid phosphatase test for the detection of alcaline phosphatase in milk, whey, cream and butter. The test allows the testing of sufficient HTST treatment of milk and related products, and the detection of raw milk in pasteurized milk. Sensitivity of the test is 3.5 mU/L.

KaryoPrep RBC Lytic Reagent

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KaryoPrep RBC Lytic Reagent combines the properties of 2 activities in one reagent. It has mucolytic activity used in the sample purification procedures. The aim of this reagent is to remove a part of mucus eventually present in some samples. Depending on the biological specimen and the appropriate procedure, the user can evaluate whether or not to use KaryoPrep RBC Lytic Reagent. It is provided in various volumes in HDPE plastic material. The HDPE material, certified by the provider, is free of BPA, phthalates and latex and does not contain, or contains less than the 0.1% of substances that can be defined as ''high concern'', identified as SVHC and listed in the candidates list in the XIV REACH annex. LBC LEAFLET

SODIUM DIRECT, UV, Enzymatic Kinetic

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PRINCIPLE Sodium is determined enzymatically via sodium dependent β--galactosidase activity with ONPG as substrate. The absorbance at 405 nm of the product O-nitrophenyl is proportional to the sodium concentration. CONTENTS MM-1050 R1: 100 mL., R2: 50 mL, Calibrator: 1 mL. (150 mmol/ l) R1 Buffer / Enzymes, Tris buffer, Cryptand, β-galactosidase R2 Buffer / Substrate Tris buffer O-nitrophenyl galactoside REAGENT PREPARATION AND STABILITY R1. Buffer/Enzymes - Ready to use R2.Diluent/Substrate - Ready to use All the reagents of the kit are stable up to the end of the indicated month and year of expiry. Store tightly closed at 2-8ºC. Do not use reagents over the expiration date. SPECIMEN Serum, plasma treated with lithium heparinate. MATERIAL REQUIRED BUT NOT PROVIDED - Spectrophotometer or colorimeter measuring at 405 nm. Matched cuvettes 1.0 cm light path. TEST PROCEDURE 1. Assay conditions: Wavelength: 405nm Cuvette: 1 cm light path Constant temperature: 37ºC / 15-25ºC 2. Adjust the instrument to zero with distilled water. 3. Pipette into a cuvette: (For cal.) R1: 1000 uL. + R2: 500 uL. + cal.: 25 uL. (For sample) R1: 1000 uL. + R2: 500 uL. + sample: 25 uL. 4. Mix and read the absorbance after 120 s (A1) and 360 s (A2). 5. Calculate: ∆A= A2 – A1. QUALITY CONTROL Control sera are recommended to monitor the performance of the procedure. If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Serum controls are recommended for internal quality control. Each laboratory should establish its own Quality Control scheme and corrective actions. REFERENCE VALUES 136 - 146 mmol/l (313 - 336 mg/dl) (These values are for orientation purpose). It is suggested that each laboratory establish its own reference range. CLINICAL SIGNIFICANCE Sodium measurements are used in the diagnosis and treatment of aldosteronism, diabetes insipidus, adrenal hyper-tension, Addison’s disease, dehydration, inappropriate antidiuretic hormone secretion, or other diseases involving electrolyte imbalance. REAGENT PERFORMANCE - Linearity: The method is linear between sodium concentrations of 80.0 and 180.0 mmol/L - Precision: Media (mmol/L), Intra series (n=20) 128.94, 155.84/Inter series (n=20) 128.94, 155.84 SD (mmol/L), Intra series (n=20) 1.57, 1.72/Inter series (n=20) 2.01, 2.56 CV (%), Intra series (n=20) 1.20, 1.10/Inter series (n=20) 1.56, 1.65 - Sensitivity: The minimum detectable concentration of sodium with an acceptable level of precision was determined as 80.0 mmol/l. - Accuracy: Results obtained using MenidiMedica reagents (y) did not show systematic differences when compared with other commercial reagent (x). The results obtained using 53 samples spanning the range 86,2 to 174.7 mmol/L were the following: Correlation coefficient (r): 0.98. Regression equation: y= 1.05x – 2.23 The results of the performance characteristics depend on the analyzer used. NOTES 1. In order to avoid contamination it is recommended to use disposable material. 2. MenidiMedica has instruction sheets for several automatic analyzers. Instructions for many of them are available on request. SODIUM DIRECT PDF

Formaldehyde Gel 10%

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YOUR SAFEST CHOICE FOR SURGICAL SPECIMEN PRESERVATION FORMALDEHYDE GEL 10%-SAFER FOR THE ENVIRONMENT AND LAB TECHNICIANS A unique formula designed for storing surgical specimens. It contains a 10% formaldehyde gel solution, which is safer and less harmful to both the environment and laboratory personnel compared to traditional liquid formaldehyde solutions. In pathology laboratories, 10% formaldehyde solutions are commonly used for preserving surgical specimens and biological tissues for diagnostic purposes. However, these solutions release harmful formaldehyde vapors that can irritate the eyes and respiratory system, cause headaches, nausea, and drowsiness. Formaldehyde vapors are also classified as carcinogens by the World Health Organization (WHO). To mitigate these risks, we introduce a novel approach: formaldehyde in a gel form. This innovative formulation retains all the excellent tissuepreserving properties of formaldehyde while significantly reducing vapor levels and strong odors. This contributes to the safety of both laboratory personnel and the environment. INTRODUCING FORMALDEHYDE GEL 10Formaldehyde Gel 10% ENG PDF Formaldehyde Gel 10% ENG PDF% - 1 lt.(Ref.No.: 70154-1000) - 5 lt.(Ref.No.:70154-5000) WHY CHOOSE FORMALDEHYDE GEL 10%? - Superior Tissue Preservation: Formaldehyde Gel 10% offers the same exceptional tissue preservation properties as traditional formaldehyde solutions. - Reduced Health Risks: Say goodbye to harmful formaldehyde vapors. Our gel formulation minimizes irritations, headaches, nausea, and drowsiness associated with traditional formaldehyde. - Environmental Responsibility: We're committed to sustainability. Formaldehyde Gel 10% is safer for the environment and complies with eco-friendly standards. - Easy to Use: Ready-to-use formulation, convenient for laboratory procedures. - Customizable Volume: Our containers come in various sizesand refill options, allowing precise volume adjustments as needed. PROTECT YOUR TEAM, PROTECT THE ENVIRONMENT Enhanced Safety Features: - Gel Consistency: Our gel formulation ensures proper specimen protection, minimizing spillage and leakage risks - Low Vapor Levels: Dramatically reduce formaldehyde vapor exposure, making the laboratory environment safer for technicians. - Odor Control: Say goodbye to strong formaldehyde odors; experience a more pleasant work environment. - pH Balanced: Formaldehyde Gel 10% maintains a neutral pH of 7 for optimal tissue preservation This product is intended for laboratory use only. Please follow all safety protocols and guidelines. MenidiMedica Biotech Greece is committed to environmental responsibility and product safety. Copyright © 2023 MenidiMedica Biotech Greece. All rights reserved.

KaryoPrep General Preservative

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KaryoPrep General Preservative is designed for use with a variety of methods, including: the manual method introduced by MenidiMedica, MenidiMedica LBC system, other commercial processors. During the processing of various biological samples, the user can refill the vial of KaryoPrep Solution for Fixation with the same amount of volume collected for diagnosis. In this way, optimal preservation of the uncollected biological specimen is obtained. It is provided in various volumes in HDPE plastic material. The HDPE material, certified by the provider, is free of BPA, phthalates and latex and does not contain, or contains less than the 0.1% of substances that can be defined as ''high concern'', identified as SVHC and listed in the candidates list in the XIV REACH annex. LBC LEAFLET

IRON CROMAZUROL Colorimetric method Endpoint

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PRINCIPLE The method is based on the properties of Chromazurol S (CAS), a chromogenic iron-binding dye, that under acidic conditions in presence of cetrimide (CTAB) forms an intense purple complex proportional to the concentration of iron present in the sample. REAGENT COMPOSITION R Chromazurol reagent. Acetate buffer, chromazurol, cetrimide, Mg2+, thiourea, Tween 20. C R:35/10 S :26-37/39-45 STORAGE AND STABILITY Store at 2-8ºC. All the kit compounds are stable until the expiry date stated on the label. Do not use reagents over the expiration date. Store the vials tightly closed, protected from light and prevented contaminations during the use. Discard if appear signs of deterioration: - Presence of particles and turbidity. - - Blank absorbance (A) at 635 nm > 0.575 in 1cm cuvette REAGENT PREPARATION The Reagent is ready-to-use. SAMPLES Serum or heparinized plasma. Centrifuge specimen as soon as possible after collection. Hemolyzed samples are rejected. Ruptured red cells falsely elevate the serum results. Iron in serum is stable for 3 weeks at 2-8ºC and for about 7 days at 20-25ºC. Freeze for longer storage. INTERFERENCES - Lipemia (intralipid >1.25 g/L) may affect the results. - Bilirubin (< 10 mg/dL) does not interfere. - Hemoglobin may affect the results. - Other drugs and substances may interfere MATERIALS REQUIRED - Photometer or colorimeter capable of measuring at 635 ± 20 nm. - Pipettes with disposable plastic tips to measure reagents and samples. - Disposable plastic tubes for the tests. PROCEDURE 1. Bring reagents and samples to room temperature. 2. Pipette into labelled test tubes: (For cal.) R: 1000 uL. + cal.: 50 uL. (For sample) R: 1000 uL. + sample: 50 uL. 3. Mix and let the tubes stand 10 minutes at 37ºC. 4. Read the absorbance (A) of the samples and the standard at 635 nm against the reagent blank. CALCULATIONS A Sample/A Standard x C Standard = ug/dL iron Samples with concentrations higher than 1000 ug/dL should be diluted 1:2 with saline and assayed again. Multiply the results by 2. If results are to be expressed as SI units apply: ug/dL x 0.179 = umol/L. REFERENCE VALUES Men = 60 - 175 ug/dL (10.7 - 31.3 umol/L) Women = 50 - 170 ug/dL (9.0 - 30.4 umol/L) Note: It is recommended that each laboratory establishes its own reference range QUALITY CONTROL The use of a standard to calculate results allows to obtain an accuracy independent of the system or instrument used. To ensure adequate quality control (QC), each run should include a set of controls (normal and abnormal) with assayed values handled as unknowns. If the values are found outside of the defined range, check the instrument, reagents and procedure. Each laboratory should establish its own Quality Control scheme and corrective actions if controls do not meet the acceptable tolerances. CLINICAL SIGNIFICANCE Following intestinal absoption of iron or erythrocyte destruction, iron ions are released into the plasma where they bind to either apotransferrin or apoferritin proteins to form transferrin and ferritin, respectively. The former helps transport iron to bone marrow for erythropoiesis; the latter stores iron in tissues, until is needed. An increase in the iron level in plasma due to rapid destruction of erythrocytes or excesive uptake of iron may also lead to iron overload. The latter causes iron deposition disorders in tissue known as hemosiderosis or hemochromatosis. Conversely, a decrease in the iron level in plasma due to malnutrition or malabsorbtion may lead to excesive depletion in iron storage, resulting in anemia such as iron-deficiency anemia. NOTES - Contamination of glassware with iron will affect the test. Use acid-washed glassware or plastic tubes. - This method may be used with different instruments. Any application to an instrument should be validated to demonstrate that results meet the performance characteristics of the method. It is recommended to validate periodically the instrument. Contact to the distributor for any question on the application method. - - Clinical diagnosis should not be made on findings of a single test result, but should integrate both clinical and laboratory data. ANALYTICAL PERFORMANCE Detection Limit: 10.10 ug/dL Linearity: Up to 1000 ug/dL Precision (expressed in ug/dL): Within-run Mean - 118.8, 204.6 SD - 0.95, 0.59 CV% - 0.81, 0.59 N - 10, 10 Between-run Mean - 118.8, 204.6 SD - 2.71, 3.71 CV% - 2.28, 1.81 N - 10, 10 Sensitivity : 1.6 mAbs / ug/dL iron. Correlation: This assay (y) was compared with a similar commercial method (x). The results were: N = 49 r = 0. 97 y = 0.97x + 0.10 The analytical performances have been generated using on automatic instrument. Results may vary depending on the instrument. IRON CROMAZUROL PDF

Sperm DNA Fragmentation Kit

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SPERM DNA FRAGMENTATION KIT UNLOCK THE FUTURE OF PARENTHOOD WITH SPERM DNA FRAGMENTATION KIT What is Sperm DNA Fragmentation Kit? The Sperm DNA Fragmentation technique can distinguish between functional and non-functional spermatozoa based on their DNA integrity, identifying which ones are viable for fertilization. This kit evolutionizes the assessment of sperm quality by complementing traditional sperm analysis. While conventional semen analysis focuses solely on sperm concentration, motility, and morphology, it overlooks a crucial parameter: the integrity of the DNA molecule. Interestingly, 15% of men classified as infertile have normal sperm analyses, underscoring the need for a more comprehensive evaluation. Sperm DNA fragmentation kit empowers medical professionals to make informed decisions about the most suitable assisted reproduction techniques for each couple. Employs a controlled DNA denaturation process to facilitate the removal of proteins from each spermatozoon. This method results in the formation of halos composed of DNA loops at the head of healthy sperm, a feature absent in sperm with damaged DNA. Key Features of Sperm DNA Fragmentation Kit - Easy & Quick Assessment: Say goodbye to complex laboratory equipment. Our kit provides a hassle-free, rapid measurement of Sperm DNA Fragmentation. - Comprehensive Insights: Complement traditional semen analysis with specific data on genetic material quality for a holistic evaluation. - Cost-Effective: Avoid expensive and frustrating IVF procedures. Assess Sperm DNA Fragmentation beforehand to guide fertility treatment. - Vital for Success: The integrity of paternal DNA is critical for both natural conception and assisted reproduction, ensuring the best chance of a viable pregnancy Who can use Sperm DNA Fragmentation Kit? Medical professionals rely on information provided by MenidiMedica Biotech solutions as an essential addition to the standard semen analysis when initiating treatment. Sperm DNA damage is a complex process influenced by various factors. Therefore, it is particularly recommended for the following groups: 1. Couples who have experienced recurrent miscarriages. 2. Couples facing unexplained infertility lasting more than six months. 3. Men aged over 40. 4. Individuals who frequently wear tight-fitting clothing. 5. Men with a history of cancer. 6. Men undergoing treatment with prescription medications. 7. Men exposed to harmful substances or toxins. 8. Men who have had urogenital infections. 9. Individuals with unhealthy lifestyle habits, including smoking, physical inactivity, poor dietary choices, or obesity. 10. Cases where the quality of embryos during subsequent cycles of egg donation is suboptimal. 11. Unexplained male infertility. Natural factors such as improper maturation and oxidative stress can lead to disruptions in spermatozoa production within the testicles, resulting in fertility challenges. Why Choose Sperm DNA Fragmentation Kit? - Accurate Evaluation: Obtain precise results with high-contrast halo images using standard bright-field microscopy after Wright staining. - Sperm Identification: Preserve sperm tails for easy differentiation from other cell types and identification of degraded sperm cells with varying halo sizes. - User-Friendly: A simple protocol involving acid treatment and lysis solution makes it easy to assess DNA integrity. - Functional vs. Non-Functional: Identify which spermatozoa are functional for fertilization and distinguish them from non-functional ones. How Does It Work? Our cutting-edge technique is based on the principle that sperm with fragmented DNA do not display the characteristic halo of dispersed DNA loops found in sperm with intact DNA after acid denaturation and nuclear protein removal. Fresh semen samples should be collected in a sterile recipient. The sperm DNA fragmentation assay should be performed immediately once the sperm sample has been obtained or thawed after cryopreservation. a. The spermatozoa are placed in an agarose microgel and evenly distributed onto a slide. b. The sample is subjected to acid denaturation and a lysis solution as part of the treatment process. c. After treatment, the sample undergoes dehydration, staining, and subsequent microscopic visualization for examination of fragmented and non-fragmented DNA. d. To calculate the percentage of sperm with fragmented DNA (SDF), count SDF sperm based on specified criteria and divide by the total sperm counted (N), then evaluate the SDF percentage in relation to established thresholds for SDF levels. Storage Conditions Upon receipt, please store the kit in a location with a temperature range between 2ºC and 30ºC, and ensure it is shielded from exposure to light. Once the kit is opened, it maintains stability for a period of 12 months. Description of kit reagents Every kit contains the necessary to perform 10 assays. The components are: - Agarose Tubes; 10 units - Denaturant Agent 1 mL screw tube - Lysis Solution, six 20 ml bottle each Sperm classification In each sample, a minimum of 300 spermatozoa should be counted, following the specified criteria for categorization: Spermatozoa without DNA fragmentation: - Spermatozoa with big halo: Sperm with halos whose width is equal to or greater than the diameter of the core (referred to as "Big halo"). - Spermatozoa with medium-sized halo: Sperm with halos sized between those with large halos and those with very small halos (referred to as "Medium halo"). - Others: Cells referred to as "others" that do not correspond to spermatozoa, distinguished by the absence of a tail. These cells must not be included in the estimation of the frequency of sperm with fragmented DNA. Spermatozoa with fragmented DNA: - Spermatozoa with small halo: Sperm displaying halos with a width equal to or smaller than 1/3 of the diameter of the core (referred to as "Small halo"). - Spermatozoa without halo: Sperm with no visible halo (referred to as "Without halo"). - Spermatozoa without halo and degraded: Sperm that lack a halo and exhibit irregular or weak staining of the core (referred to as "Degraded"). Order Your Sperm DNA Fragmentation Kit Today! Make the right choices on the path to parenthood. The Sperm DNA Fragmentation Kit empowers medical professionals with the knowledge needed for fertility journey. Contact us now to order your kit. We strive for a better, safer world for us and our children. SDF-ENG-PDF

KaryoPrep CellWhole Reagent

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KaryoPrep CellWhole Reagent is designed and developed for urine biological samples which have few cells for examination. One drop from KaryoPrep Cell Whole Reagent on a glass slide is enough for stamping and processing of this kind of samples. It is provided in various volumes in HDPE plastic material. The HDPE material, certified by the provider, is free of BPA, phthalates and latex and does not contain, or contains less than the 0.1% of substances that can be defined as ''high concern'', identified as SVHC and listed in the candidates list in the XIV REACH annex. LBC LEAFLET

Bromate Direct

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QUANTITATIVE DETECTION OF BROMATE IN BREAD Potassium bromate, or simply called bromate, is an oxidiser used to strengthen dough and enhance its elasticity. This helps bake uniform and whitened bread. However, excessive use of potassium bromate results in residual concentrations in bread having potentially harmful effects. Some people who ingested large amounts of bromate had gastrointestinal symptoms such as nausea, vomiting, diarrhea and abdominal pain. Some individuals who ingested high concentrations of bromate also experienced kidney effects, nervous system effects and hearing loss. European bread lacks a specific ingredient: Potassium bromate. Food makers in the United States regularly use this chemical compound to strengthen dough. In fact, this additive is present in more than 100 products. But, Europe, China, and India have banned Potassium bromate due to concerns that it may be a carcinogen. CHARACTERISTICS Reference: 82906 Linearity range: 0.5 - 50 ug/mL. bromate content Presentation: Chromogen Activator R1 - 5 mL., Substrate R2 - 2 mL. Sample Matrices: bread, water Expiry Date: 24 months *The kit must be stored at 2°-8°C HOW TO USE Method: Quantitative, Endpoint Wavelength: 505 nm Sample preparation: Collect 1 gr. of bread, cut and mix in 20 mL. distilled water with magnetic stirrer. Filter through Whatman 41 filter. Blank: Reagent Procedure: 1. Read Blank 2. Transfer 880 uL. sample filtrate into a cuvette 3. Add 100 uL. R1 and mix 4. Add 20 uL. R2, shake in vortex for 1' 5. Read results The color is stable for 30' BROMATE DIRECT ENG PDF

TETHYS: UNIQUE RAPID CARIES DETECTION TEST

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THE FUTURE OF ORAL HEALTH: UNIQUE RAPID CARIES DETECTION TEST JOIN THE ‘BETTER ORAL HEALTH MOVEMENT FOR ALL’ The majority of participants in our clinical trial (90%) recognised the importance of early detection of caries. Boost your oral health and enjoy a healthier smile. TAKE CHARGE OF YOUR ORAL HEALTH Globally, in 2019, 3.09 billion new cases of caries in permanent teeth were recorded (an increase of 48.00%), as well as 2.03 billion existing cases of caries (an increase of 46.07%), compared to 1990. Among adolescents aged 12 to 19 years, more than half (57%) had at least some dental decay from caries in their permanent teeth. In adults aged 20 years and older, 90% had at least one dental decay from dental caries. EARLY DETECTION FOR A HEALTHIER SMILE Tooth decay is a silent threat to your oral health, but with our revolutionary TETHYS rapid caries detection test, you can now detect it early and take control of your dental wellbeing. WHY CHOOSE THE RAPID CARIES DETECTION TEST? Product of cooperation between MenidiMedica Biotech Greece and the University of West Attica. TETHYS diagnostic test for valid, early detection of dental caries, ensuring oral hygiene. - Fast and reliable: you get results within minutes, allowing early detection of caries even in its early stages. - Accurate: Our test has high sensitivity and specificity, ensuring accurate detection of caries. - Convenient: No invasive procedures or specialized equipment are required. A simple swab and a vial is all that is needed. - Safe: The test is safe and non-invasive, making it suitable for all ages. - Proven results: Backed by clinical trials, our test has shown excellent performance. HOW IT WORKS? - Open the sterile wooden swab. - Brush your teeth as usual. - Open the vial of single reagent and rinse the head of the swab in the vial. - Break off the wooden swab head and place it in the vial - Close the vial and shake gently for 5 seconds. - Place the vial in your pocket for 30 minutes. - Check for a colour change in the reagent: a. Magenta for a positive result. Have caries in progress b. Pink or transparent clear for a positive result. You have caries and a decaying tooth c. Purple for a negative result. You do not have caries DETECT-PREVENT-RESIST Don't let tooth decay develop into a painful problem. With the TETHYS quick caries test, you take control of your oral health in your own hands. CLINICAL TRIAL RESULTS - Sensitivity: 98,39% - Specificity: 100.00% - Positive predictive value (PPV): 100.00% - Negative predictive value (NPV): 99.19% - Accuracy: 99.46% tethys eng pdf

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