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MenidiMedica

Medical Instruments, Tools & Supplies in Etoloakarnania in Greece.
Our highly experienced and committed team focus all their energy to deliver what our customers demand at very competitive prices, ensuring your business success.

Postal address
Menidi Aetolias & Akarnanias
Zip code: 30016, Greece

Customer Support

T +30 2681088000
Μ +30 6937115868
Μ +30 6976292146
menidimedica@gmail.com

Business Hours
From Monday through Friday
08:00 – 17:00 (EET)

INFORMATION
MenidiMedica © 2009 – 2025. All Rights Reserved.

KaryoPrep Solution for Fixation

KaryoPrep Solution for Fixation is the fixative liquid specially dedicated to the preservation of cytological samples. It is designed, developed and produced by MenidiMedica Greece and it can be used with all kinds of biological samples. KaryoPrep Solution for Fixation is designed for use with the manual method introduced by MenidiMedica or with MenidiMedica LBC system or other commercial processors. It serves as a transport, preservative, and antibacterial medium for the processed biological samples, including certain tests for Human Papilloma Virus (HPV) and other sexually transmitted infections. It has no haemolytic activity in order to ensure conservation of red blood cells, eventually present in urinary samples, to make them visible and diagnosable by the pathologist during the specimen analysis. In case the user wishes elimination of mucus or red blood cells (absence of artifacts), KaryoPrep RBC Lytic Reagent must be applied to the KaryoPrep Solution for Fixation. KaryoPrep Solution for Fixation is a saline solution based on ethanol, which ensures the perfect cytological material fixation and a safe operator's handling at the same time. It guarantees the morphological preservation of cytological samples for at least 5 years at room temperature. The genetic material of the collected samples is stored and available for molecular biology in-depth analysis, up until 5 years after collection. For optimal samples fixation, it is recommended to use unexpired vials of KaryoPrep Solution for Fixation. The vials are ready-to-use and are stable for 60 months at room temperature. Karyoprep Solution for fixation is supplied in different packaging, ranging from 10 mL. prefilled collection vials, to 1 or 5 Lt. - bottles approved for air transport. The vials are made of a special transparent plastic material, characterized by peculiar optical transmittance technical specifications, allowing them to be used with the MenidiMedica LBC fitting system and with various other platform systems. The 1 Lt - formats are supplied in HDPE plastic material bottles. HDPE material, certified by the provider, is free of BPA, phthalates and latex and does not contain or contains less than the 0.1|% of substances that can be defined as ''high concern'', identified as SVHC and listed in the candidates list in the XIV REACH annex. The 5 Lt - formats are provided in stackable plastic tanks, UN/ADR approved for land, air and sea transport of dangerous liquid products, according to UN Legislation. LBC LEAFLET

Total Polyphenols Direct

Quantitative Determination of total polyphenols in edible oils, wine and food Description The antioxidant capacity of polyphenols plays an important role in the stability of olive oil, as there is a correlation between the amount of total polyphenols and resistance to oxidation. Polyphenols have a protective effect on the cells of the human body because they oppose the negative effects of free radicals. These polyphenols in olive oil act as natural antioxidants. The amount of polyphenols decreases during olive oil extraction, so the test can be used to optimize the processing. Olive oil is rich in polyphenols, which form its "polar fraction" and prevent its self-oxidation, thus giving it its excellent thermal stability and contributing to its characteristic aroma and taste. The main ones are: tyrosol, hydroxytyrosol, oleuropein and protocatechuic, gallic, vanillic, vanillic, p-hydroxybenzoic, syringic, 4-hydroxyphenyl acetate, omavanillic, quinamic, o-coumaric, p-coumaric, caffeic, ferulic and sinoacetic acids. The main antioxidants of olive oil are considered to be tocopherols, the main representative being alpha-tocopherol, and phenolic compounds. Vitamin E with its parent compound tocol consists of two series of compounds: tocopherols and tocotrienols. The tocopherols are divided into a-tocopherol, b-tocopherol, c-tocopherol, and d-tocopherol, while the tocotrienols are divided into a-, b-, c-, and d-tocotrienol. The total biological activity of vitamin E is mainly due to the presence of αtocopherol. It is the alpha-tocopherol that exerts the greatest biological activity in the human body and is the reference for the action of the other forms of vitamin E. All tocopherols are natural antioxidants of oils, since they have an antioxidant activity that increases from a to d. The stability of olive oil to oxidation is largely due to the presence of tocopherols, which are easily oxidised. A-tocopherol is traditionally considered the main antioxidant in olive oil. It constitutes about 90% of all tocopherols and normally ranges from 100 to 300 ppm (Blekas et al., 1995; Psomiadou and Tsimidou, 1998). c-Tocopherol constitutes about 8% of all tocopherols in olive oil, while b-, d-tocopherols are only present in trace amounts (Andrikopoulos et al., 1989). Vitamin E is an important natural antioxidant of oils, since it inhibits the oxidation of their fatty substances (triglycerides), and protects olive oil from peroxidation and the free radical propagation mechanism. Package contents: 1 x 5 ml R1а, 3 x 17 ml R1b, 1 x 7.5 ml R2 Number of tests: 50 tests Ref.: 89001 Shelf life: 24 months from date of manufacture Storage & Stability: 2-8°C Sample collection instructions No preparation of olive oil sample is required. Preparation of reagents *WR1: Mix 100 μl R1a with 1000 μl R1b and shake gently for 3 seconds R1а - Mixing with R1b is required R1b - Mixing with R1a is required R2 - Ready for use Procedure - Wavelength: 700 nm on the Electra m2 Unified Analyzer - Temperature: 30°C (25°C – 37°C) - Method: FIXED TIME - Fitting: LINEAR - Select the T.PPS parameter from the test menu - ZERO: WR1 500 uL. + 10 uL. deionized water, Press OK. - ADD REAGENT R1 : WR1: 500 uL. Press OK. - ADD SAMPLE: 10 uL.Press OK. - Wait 180 seconds. - Measurement of absorbance A1. ADD REAGENT R2: 150 uL. Press OK. - Wait 300 seconds. - Absorbance measurement A2. DA = A2-A1 (total polyphenol concentration expressed in mg/l.) Note: A 5-point calibration of 0, 125, 250, 500, 1000 (mg/l) is recommended. Reference values Linearity: 0-1000 mg/l. Note: For samples that the analyzer gives you an UPPER LIMIT value, then you should dilute your sample with OLIVE OIL DILUTOR (Ref. 89377, sold separately by MenidiMedica Biotech Greece) in a ratio of 1:1 or 1:5 and multiply the result given by the analyzer by 1 or 5. Safety measures The components of elean morian polyphenols pose no health risk when used in accordance with standard laboratory practices and the procedures in this insert. For further safety instructions, refer to the Safety Data Sheet (SDS). Notes A 4-level calibration kit is available by MenidiMedica Biotech Greece (Recommended for calibration on automatic instruments). Total Polyphenols Direct Quantitative method in fats, oils, bakery products, nuts, dried fruits IFU ENG PDF

KaryoPrep Fixative

KaryoPrep Fixative is the protective alcoholic solution designed to protect cells from air contact during the drying phase of the slides prepared with MenidiMedica cytology processors, or with the manual method. Compatible with the reagents of other liquid based cytology technology methods, it can be implemented, as a universal fixative, into the sample processing of competition. It is supplied in various volumes in HDPE plastic material. The HDPE material, certified by the provider, is free of BPA, phthalates and latex and does not contain or contains less than the 0.1% of substances that can be defined as ''high concern'', identified as SVHC and listed in the candidates list in the XIV REACH annex. LBC LEAFLET

L-Ascorbic Acid Direct (Vitamin C)

QUANTITATIVE DETECTION OF L-ASCORBIC ACID Ascorbic acid is a nutrient the human body needs in small amounts to function, and it can help prevent cell damage caused by free radicals— unstable molecules that can damage cells. It can also help the human body fight bacterial infections. Cosmetics and other personal care products may include less acidic forms of ascorbic acid, which can act as antioxidants to slow product deterioration. The FDA states that ascorbic acid is a generally recognized as safe substance for use as a chemical preservative in foods and as a nutrient or dietary supplement. The Cosmetic Ingredient Review states that ascorbic acid and its salts are safe for use in cosmetic and personal care products. According to the U.S. National Cancer Institute, ascorbic acid can help the human body fight bacterial infections and help form collagen, an important protein in fibrous tissue, teeth, bones, skin and capillaries. CHARACTERISTICS Reference: 82905 Linearity range: 0.2-50 mg/dL. L-ascorbic acid content Presentation: Chromogen Activator R1: 20 mL., Substrate R2: 20 mL. Sample Matrices: food, fruits, cosmetics, juices, pharmaceuticals Expiry Date: 24 months *The kit must be stored at 2°-8°C HOW TO USE Method: Quantitative, Endpoint Wavelength: 545 nm Blank: Water Procedure: 1. Read Blank 2. Transfer 200 uL. R1 into a cuvette 3. Add 200 uL. R2 into the cuvette 4. Add 50 uL. sample into the cuvette and mix incubate for 30'' 6. Read results The color is stable for 40' L-ASCORBIC ACID DIRECT ENG PDF

Lactopast Biomedix Fast Phosphatase

Instructions for use  We have researched and developed a fast qualitative test of milk pasteurization with no need of any instrumental or incubational means with the name LACTOPAST BIOMEDIX. It is a rapid phosphatase test for the detection of alcaline phosphatase in milk, whey, cream and butter. The test allows the testing of sufficient HTST treatment of milk and related products, and the detection of raw milk in pasteurized milk. Sensitivity of the test is 3.5 mU/L.

KaryoPrep RBC Lytic Reagent

KaryoPrep RBC Lytic Reagent combines the properties of 2 activities in one reagent. It has mucolytic activity used in the sample purification procedures. The aim of this reagent is to remove a part of mucus eventually present in some samples. Depending on the biological specimen and the appropriate procedure, the user can evaluate whether or not to use KaryoPrep RBC Lytic Reagent. It is provided in various volumes in HDPE plastic material. The HDPE material, certified by the provider, is free of BPA, phthalates and latex and does not contain, or contains less than the 0.1% of substances that can be defined as ''high concern'', identified as SVHC and listed in the candidates list in the XIV REACH annex. LBC LEAFLET

SODIUM DIRECT, UV, Enzymatic Kinetic

PRINCIPLE Sodium is determined enzymatically via sodium dependent β--galactosidase activity with ONPG as substrate. The absorbance at 405 nm of the product O-nitrophenyl is proportional to the sodium concentration. CONTENTS MM-1050 R1: 100 mL., R2: 50 mL, Calibrator: 1 mL. (150 mmol/ l) R1 Buffer / Enzymes, Tris buffer, Cryptand, β-galactosidase R2 Buffer / Substrate Tris buffer O-nitrophenyl galactoside REAGENT PREPARATION AND STABILITY R1. Buffer/Enzymes - Ready to use R2.Diluent/Substrate - Ready to use All the reagents of the kit are stable up to the end of the indicated month and year of expiry. Store tightly closed at 2-8ºC. Do not use reagents over the expiration date. SPECIMEN Serum, plasma treated with lithium heparinate. MATERIAL REQUIRED BUT NOT PROVIDED - Spectrophotometer or colorimeter measuring at 405 nm. Matched cuvettes 1.0 cm light path. TEST PROCEDURE 1. Assay conditions: Wavelength: 405nm Cuvette: 1 cm light path Constant temperature: 37ºC / 15-25ºC 2. Adjust the instrument to zero with distilled water. 3. Pipette into a cuvette: (For cal.) R1: 1000 uL. + R2: 500 uL. + cal.: 25 uL. (For sample) R1: 1000 uL. + R2: 500 uL. + sample: 25 uL. 4. Mix and read the absorbance after 120 s (A1) and 360 s (A2). 5. Calculate: ∆A= A2 – A1. QUALITY CONTROL Control sera are recommended to monitor the performance of the procedure. If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Serum controls are recommended for internal quality control. Each laboratory should establish its own Quality Control scheme and corrective actions. REFERENCE VALUES 136 - 146 mmol/l (313 - 336 mg/dl) (These values are for orientation purpose). It is suggested that each laboratory establish its own reference range. CLINICAL SIGNIFICANCE Sodium measurements are used in the diagnosis and treatment of aldosteronism, diabetes insipidus, adrenal hyper-tension, Addison’s disease, dehydration, inappropriate antidiuretic hormone secretion, or other diseases involving electrolyte imbalance. REAGENT PERFORMANCE - Linearity: The method is linear between sodium concentrations of 80.0 and 180.0 mmol/L - Precision: Media (mmol/L), Intra series (n=20) 128.94, 155.84/Inter series (n=20) 128.94, 155.84 SD (mmol/L), Intra series (n=20) 1.57, 1.72/Inter series (n=20) 2.01, 2.56 CV (%), Intra series (n=20) 1.20, 1.10/Inter series (n=20) 1.56, 1.65 - Sensitivity: The minimum detectable concentration of sodium with an acceptable level of precision was determined as 80.0 mmol/l. - Accuracy: Results obtained using MenidiMedica reagents (y) did not show systematic differences when compared with other commercial reagent (x). The results obtained using 53 samples spanning the range 86,2 to 174.7 mmol/L were the following: Correlation coefficient (r): 0.98. Regression equation: y= 1.05x – 2.23 The results of the performance characteristics depend on the analyzer used. NOTES 1. In order to avoid contamination it is recommended to use disposable material. 2. MenidiMedica has instruction sheets for several automatic analyzers. Instructions for many of them are available on request. SODIUM DIRECT PDF

Formaldehyde Gel 10%

YOUR SAFEST CHOICE FOR SURGICAL SPECIMEN PRESERVATION FORMALDEHYDE GEL 10%-SAFER FOR THE ENVIRONMENT AND LAB TECHNICIANS A unique formula designed for storing surgical specimens. It contains a 10% formaldehyde gel solution, which is safer and less harmful to both the environment and laboratory personnel compared to traditional liquid formaldehyde solutions. In pathology laboratories, 10% formaldehyde solutions are commonly used for preserving surgical specimens and biological tissues for diagnostic purposes. However, these solutions release harmful formaldehyde vapors that can irritate the eyes and respiratory system, cause headaches, nausea, and drowsiness. Formaldehyde vapors are also classified as carcinogens by the World Health Organization (WHO). To mitigate these risks, we introduce a novel approach: formaldehyde in a gel form. This innovative formulation retains all the excellent tissuepreserving properties of formaldehyde while significantly reducing vapor levels and strong odors. This contributes to the safety of both laboratory personnel and the environment. INTRODUCING FORMALDEHYDE GEL 10Formaldehyde Gel 10% ENG PDFFormaldehyde Gel 10% ENG PDF% - 1 lt.(Ref.No.: 70154-1000) - 5 lt.(Ref.No.:70154-5000) WHY CHOOSE FORMALDEHYDE GEL 10%? - Superior Tissue Preservation: Formaldehyde Gel 10% offers the same exceptional tissue preservation properties as traditional formaldehyde solutions. - Reduced Health Risks: Say goodbye to harmful formaldehyde vapors. Our gel formulation minimizes irritations, headaches, nausea, and drowsiness associated with traditional formaldehyde. - Environmental Responsibility: We're committed to sustainability. Formaldehyde Gel 10% is safer for the environment and complies with eco-friendly standards. - Easy to Use: Ready-to-use formulation, convenient for laboratory procedures. - Customizable Volume: Our containers come in various sizesand refill options, allowing precise volume adjustments as needed. PROTECT YOUR TEAM, PROTECT THE ENVIRONMENT Enhanced Safety Features: - Gel Consistency: Our gel formulation ensures proper specimen protection, minimizing spillage and leakage risks - Low Vapor Levels: Dramatically reduce formaldehyde vapor exposure, making the laboratory environment safer for technicians. - Odor Control: Say goodbye to strong formaldehyde odors; experience a more pleasant work environment. - pH Balanced: Formaldehyde Gel 10% maintains a neutral pH of 7 for optimal tissue preservation This product is intended for laboratory use only. Please follow all safety protocols and guidelines. MenidiMedica Biotech Greece is committed to environmental responsibility and product safety. Copyright © 2023 MenidiMedica Biotech Greece. All rights reserved.

KaryoPrep General Preservative

KaryoPrep General Preservative is designed for use with a variety of methods, including: the manual method introduced by MenidiMedica, MenidiMedica LBC system, other commercial processors. During the processing of various biological samples, the user can refill the vial of KaryoPrep Solution for Fixation with the same amount of volume collected for diagnosis. In this way, optimal preservation of the uncollected biological specimen is obtained. It is provided in various volumes in HDPE plastic material. The HDPE material, certified by the provider, is free of BPA, phthalates and latex and does not contain, or contains less than the 0.1% of substances that can be defined as ''high concern'', identified as SVHC and listed in the candidates list in the XIV REACH annex. LBC LEAFLET

IRON CROMAZUROL Colorimetric method Endpoint

PRINCIPLE The method is based on the properties of Chromazurol S (CAS), a chromogenic iron-binding dye, that under acidic conditions in presence of cetrimide (CTAB) forms an intense purple complex proportional to the concentration of iron present in the sample. REAGENT COMPOSITION R Chromazurol reagent. Acetate buffer, chromazurol, cetrimide, Mg2+, thiourea, Tween 20. C R:35/10 S :26-37/39-45 STORAGE AND STABILITY Store at 2-8ºC. All the kit compounds are stable until the expiry date stated on the label. Do not use reagents over the expiration date. Store the vials tightly closed, protected from light and prevented contaminations during the use. Discard if appear signs of deterioration: - Presence of particles and turbidity. - - Blank absorbance (A) at 635 nm > 0.575 in 1cm cuvette REAGENT PREPARATION The Reagent is ready-to-use. SAMPLES Serum or heparinized plasma. Centrifuge specimen as soon as possible after collection. Hemolyzed samples are rejected. Ruptured red cells falsely elevate the serum results. Iron in serum is stable for 3 weeks at 2-8ºC and for about 7 days at 20-25ºC. Freeze for longer storage. INTERFERENCES - Lipemia (intralipid >1.25 g/L) may affect the results. - Bilirubin (< 10 mg/dL) does not interfere. - Hemoglobin may affect the results. - Other drugs and substances may interfere MATERIALS REQUIRED - Photometer or colorimeter capable of measuring at 635 ± 20 nm. - Pipettes with disposable plastic tips to measure reagents and samples. - Disposable plastic tubes for the tests. PROCEDURE 1. Bring reagents and samples to room temperature. 2. Pipette into labelled test tubes: (For cal.) R: 1000 uL. + cal.: 50 uL. (For sample) R: 1000 uL. + sample: 50 uL. 3. Mix and let the tubes stand 10 minutes at 37ºC. 4. Read the absorbance (A) of the samples and the standard at 635 nm against the reagent blank. CALCULATIONS A Sample/A Standard x C Standard = ug/dL iron Samples with concentrations higher than 1000 ug/dL should be diluted 1:2 with saline and assayed again. Multiply the results by 2. If results are to be expressed as SI units apply: ug/dL x 0.179 = umol/L. REFERENCE VALUES Men = 60 - 175 ug/dL (10.7 - 31.3 umol/L) Women = 50 - 170 ug/dL (9.0 - 30.4 umol/L) Note: It is recommended that each laboratory establishes its own reference range QUALITY CONTROL The use of a standard to calculate results allows to obtain an accuracy independent of the system or instrument used. To ensure adequate quality control (QC), each run should include a set of controls (normal and abnormal) with assayed values handled as unknowns. If the values are found outside of the defined range, check the instrument, reagents and procedure. Each laboratory should establish its own Quality Control scheme and corrective actions if controls do not meet the acceptable tolerances. CLINICAL SIGNIFICANCE Following intestinal absoption of iron or erythrocyte destruction, iron ions are released into the plasma where they bind to either apotransferrin or apoferritin proteins to form transferrin and ferritin, respectively. The former helps transport iron to bone marrow for erythropoiesis; the latter stores iron in tissues, until is needed. An increase in the iron level in plasma due to rapid destruction of erythrocytes or excesive uptake of iron may also lead to iron overload. The latter causes iron deposition disorders in tissue known as hemosiderosis or hemochromatosis. Conversely, a decrease in the iron level in plasma due to malnutrition or malabsorbtion may lead to excesive depletion in iron storage, resulting in anemia such as iron-deficiency anemia. NOTES - Contamination of glassware with iron will affect the test. Use acid-washed glassware or plastic tubes. - This method may be used with different instruments. Any application to an instrument should be validated to demonstrate that results meet the performance characteristics of the method. It is recommended to validate periodically the instrument. Contact to the distributor for any question on the application method. - - Clinical diagnosis should not be made on findings of a single test result, but should integrate both clinical and laboratory data. ANALYTICAL PERFORMANCE Detection Limit: 10.10 ug/dL Linearity: Up to 1000 ug/dL Precision (expressed in ug/dL): Within-run Mean - 118.8, 204.6 SD - 0.95, 0.59 CV% - 0.81, 0.59 N - 10, 10 Between-run Mean - 118.8, 204.6 SD - 2.71, 3.71 CV% - 2.28, 1.81 N - 10, 10 Sensitivity : 1.6 mAbs / ug/dL iron. Correlation: This assay (y) was compared with a similar commercial method (x). The results were: N = 49 r = 0. 97 y = 0.97x + 0.10 The analytical performances have been generated using on automatic instrument. Results may vary depending on the instrument. IRON CROMAZUROL PDF

Sperm DNA Fragmentation Kit

SPERM DNA FRAGMENTATION KIT UNLOCK THE FUTURE OF PARENTHOOD WITH SPERM DNA FRAGMENTATION KIT What is Sperm DNA Fragmentation Kit? The Sperm DNA Fragmentation technique can distinguish between functional and non-functional spermatozoa based on their DNA integrity, identifying which ones are viable for fertilization. This kit evolutionizes the assessment of sperm quality by complementing traditional sperm analysis. While conventional semen analysis focuses solely on sperm concentration, motility, and morphology, it overlooks a crucial parameter: the integrity of the DNA molecule. Interestingly, 15% of men classified as infertile have normal sperm analyses, underscoring the need for a more comprehensive evaluation. Sperm DNA fragmentation kit empowers medical professionals to make informed decisions about the most suitable assisted reproduction techniques for each couple. Employs a controlled DNA denaturation process to facilitate the removal of proteins from each spermatozoon. This method results in the formation of halos composed of DNA loops at the head of healthy sperm, a feature absent in sperm with damaged DNA. Key Features of Sperm DNA Fragmentation Kit - Easy & Quick Assessment: Say goodbye to complex laboratory equipment. Our kit provides a hassle-free, rapid measurement of Sperm DNA Fragmentation. - Comprehensive Insights: Complement traditional semen analysis with specific data on genetic material quality for a holistic evaluation. - Cost-Effective: Avoid expensive and frustrating IVF procedures. Assess Sperm DNA Fragmentation beforehand to guide fertility treatment. - Vital for Success: The integrity of paternal DNA is critical for both natural conception and assisted reproduction, ensuring the best chance of a viable pregnancy Who can use Sperm DNA Fragmentation Kit? Medical professionals rely on information provided by MenidiMedica Biotech solutions as an essential addition to the standard semen analysis when initiating treatment. Sperm DNA damage is a complex process influenced by various factors. Therefore, it is particularly recommended for the following groups: 1. Couples who have experienced recurrent miscarriages. 2. Couples facing unexplained infertility lasting more than six months. 3. Men aged over 40. 4. Individuals who frequently wear tight-fitting clothing. 5. Men with a history of cancer. 6. Men undergoing treatment with prescription medications. 7. Men exposed to harmful substances or toxins. 8. Men who have had urogenital infections. 9. Individuals with unhealthy lifestyle habits, including smoking, physical inactivity, poor dietary choices, or obesity. 10. Cases where the quality of embryos during subsequent cycles of egg donation is suboptimal. 11. Unexplained male infertility. Natural factors such as improper maturation and oxidative stress can lead to disruptions in spermatozoa production within the testicles, resulting in fertility challenges. Why Choose Sperm DNA Fragmentation Kit? - Accurate Evaluation: Obtain precise results with high-contrast halo images using standard bright-field microscopy after Wright staining. - Sperm Identification: Preserve sperm tails for easy differentiation from other cell types and identification of degraded sperm cells with varying halo sizes. - User-Friendly: A simple protocol involving acid treatment and lysis solution makes it easy to assess DNA integrity. - Functional vs. Non-Functional: Identify which spermatozoa are functional for fertilization and distinguish them from non-functional ones. How Does It Work? Our cutting-edge technique is based on the principle that sperm with fragmented DNA do not display the characteristic halo of dispersed DNA loops found in sperm with intact DNA after acid denaturation and nuclear protein removal. Fresh semen samples should be collected in a sterile recipient. The sperm DNA fragmentation assay should be performed immediately once the sperm sample has been obtained or thawed after cryopreservation. a. The spermatozoa are placed in an agarose microgel and evenly distributed onto a slide. b. The sample is subjected to acid denaturation and a lysis solution as part of the treatment process. c. After treatment, the sample undergoes dehydration, staining, and subsequent microscopic visualization for examination of fragmented and non-fragmented DNA. d. To calculate the percentage of sperm with fragmented DNA (SDF), count SDF sperm based on specified criteria and divide by the total sperm counted (N), then evaluate the SDF percentage in relation to established thresholds for SDF levels. Storage Conditions Upon receipt, please store the kit in a location with a temperature range between 2ºC and 30ºC, and ensure it is shielded from exposure to light. Once the kit is opened, it maintains stability for a period of 12 months. Description of kit reagents Every kit contains the necessary to perform 10 assays. The components are: - Agarose Tubes; 10 units - Denaturant Agent 1 mL screw tube - Lysis Solution, six 20 ml bottle each Sperm classification In each sample, a minimum of 300 spermatozoa should be counted, following the specified criteria for categorization: Spermatozoa without DNA fragmentation: - Spermatozoa with big halo: Sperm with halos whose width is equal to or greater than the diameter of the core (referred to as "Big halo"). - Spermatozoa with medium-sized halo: Sperm with halos sized between those with large halos and those with very small halos (referred to as "Medium halo"). - Others: Cells referred to as "others" that do not correspond to spermatozoa, distinguished by the absence of a tail. These cells must not be included in the estimation of the frequency of sperm with fragmented DNA. Spermatozoa with fragmented DNA: - Spermatozoa with small halo: Sperm displaying halos with a width equal to or smaller than 1/3 of the diameter of the core (referred to as "Small halo"). - Spermatozoa without halo: Sperm with no visible halo (referred to as "Without halo"). - Spermatozoa without halo and degraded: Sperm that lack a halo and exhibit irregular or weak staining of the core (referred to as "Degraded"). Order Your Sperm DNA Fragmentation Kit Today! Make the right choices on the path to parenthood. The Sperm DNA Fragmentation Kit empowers medical professionals with the knowledge needed for fertility journey. Contact us now to order your kit. We strive for a better, safer world for us and our children. SDF-ENG-PDF

KaryoPrep CellWhole Reagent

KaryoPrep CellWhole Reagent is designed and developed for urine biological samples which have few cells for examination. One drop from KaryoPrep Cell Whole Reagent on a glass slide is enough for stamping and processing of this kind of samples. It is provided in various volumes in HDPE plastic material. The HDPE material, certified by the provider, is free of BPA, phthalates and latex and does not contain, or contains less than the 0.1% of substances that can be defined as ''high concern'', identified as SVHC and listed in the candidates list in the XIV REACH annex. LBC LEAFLET
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